55份湖南省白菜型油菜遗传多样性分析  

Genetic diversity analysis on 55 B. campestris L. from Hunan province in China

作　　者：陈碧云[1] 伍晓明[1] 许鲲[1] 王汉中[1] 李响枝[1] 

机构地区：[1]中国农业科学院油料作物研究所农业部油料作物遗传改良开放重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2004年第3期10-14,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金重点项目(39830270)

摘　　要：用20个随机引物对55份来自湖南白菜型油菜进行遗传多样性分析,共获得322个RAPD标记位点,其中220个位点具有多态性,多态率达68.3%。利用322个标记位点,用UPMGA法建立了湖南省55份白菜型油菜的亲缘关系聚类图,结果显示,总体上亲缘关系较近的品种在地理分布上靠得较近,聚类将55个品种分为Ⅰ、Ⅱ、Ⅲ簇,第Ⅰ簇49个品种,第Ⅱ簇2个品种,第Ⅲ簇4个品种。来自泸溪县的泸溪甜油菜和溆浦县的溆浦油菜之间的遗传距离最小(0.0398),亲缘关系最近。用SPSS统计软件对农艺性状聚类,结果分为A、B两个大簇,分别由27和28个品种组成。对来自同一县的多个品种的RAPD结果进行了遗传多样性指数分析,除汉寿县白菜型油菜的Shannon指数小于Simpson指数外,其他13个县的白菜型油菜Shannon指数均大于Simpson指数;新晃县白菜型油菜Shannon指数和Simpson指数均为最高,洞口县白菜型油菜Shannon指数和Simpson指数均为最低。55 samples were amplified with 20 random primers, 322 RAPD alleles were got .There were 220 polymorphic alleles in the 322 RAPD alleles, polymorphic rate was 68.3%. Using 322 marker alleles with UPMGA method, dendrogram on 55 B.campestris L. from Hunan province was constructed. Genetic distance among 55 varities showed that germplasm of near relationship was near in geographic distribution. It also revealed that the genetic distance between Luxi sweat rapeseed and Xupu rapeseed was the smallest, 0.0398. And their relationship was the closest. RAPD dendrogram was consisted of three groups of Ⅰand Ⅱand Ⅲ, which were made up of 49, 2,4 varieties respectively. Dendrogram of agronomic trait on 55 B.campestris L. was consisted of two groups of A and B, which were made up of 27 and 28 varieties respectively. Genetic diversity index showed that Shannon-Weaver index and Simpson index of B.camptris from Xinhuang county was the highest among 14 counties and that of B.campetris from Dongkou county was the lowest. The result of two indexes was consistent except Hanshou county.

关 键 词：湖南 白菜型油菜 遗传多样性 RAPD标记 农艺性状 

分 类 号：S565.4[农业科学—作物学]