合肥地区发生的西瓜花叶病的病原鉴定  

Identification of Watermelon mosaic disease in Hefei

作　　者：张大伟[1,2] 李世访 范卫红[1] 成卓敏[2] 王杰[1] 

机构地区：[1]安徽农业大学植物病理教研室,合肥230036 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100094

出　　处：《植物病理学报》2004年第5期474-476,共3页Acta Phytopathologica Sinica

基　　金：安徽省自然科学基金资助项目 ( 0 10 1140 3)

摘　　要：从合肥市郊区的西瓜病叶上分离出一病毒分离物 ,该分离物的热钝化温度为 60～ 65℃ ,稀释终点为 1 0 - 4～ 1 0 - 5,寄主体外存活期为 2 0～ 2 3 d,电镜观察病毒粒子约 75 0 nm× 1 3 nm。参考已发表的小西葫芦黄化花叶病毒 ( Zucchini yellowmosaicvirus,ZYMV) CP基因序列设计引物 ,RT-PCR扩增得到 CP基因片段。将该 CP片段克隆到 p GEM-3 Zf( + )中 ,测序结果表明 ,该 CP基因全长 840 nt,共编码 2 79个氨基酸 ,与国内报道的 ZYMV CP基因的核苷酸序列同源性为 83 .0 %～97.3 % ,氨基酸序列的同源性为 91 .8%～ 99.8% ,证明该分离物为 ZYMV。A virus isolate, HF WX, was isolated from infected watermelon in Hefei suburbs. Its dilution end point (DEP) was 10 -4 -10 -5 ,thermal inactivation point (TIP) was 60-65℃ and longevity in vitro was 20-23 d (20-22℃ ). The purified virus particles observed under electronic microscope were about 750 nm×13 nm. Synthesizing the primers according to the known Zucchini yellow mosaic virus (ZYMV) coat protein gene sequence, the CP gene was cloned by means of RT PCR amplification. Then it was inserted into the vector pGEM 3Zf(+). The CP gene was 840 nucleotides determined by sequen cing. Compared with the published sequences inland,it shared identities of 83.0%-97.3% in nucleotide acids and 91.8%-99.8% in amino acids respectively. Based on the above results, it is concluded that this isolate belongs to ZYMV.

关 键 词：合肥市 西瓜 花叶病 病原鉴定 小西葫芦黄化花叶病毒 CP基因 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]