欧洲黑杨转基因稳定性及对土壤微生物的影响  

Transgene Stability of Transgenic Populus nigra and Its Effects on Soil Microorganism

作　　者：胡建军[1] 张蕴哲[1] 卢孟柱[1] 张建国[1] 张守攻[1] 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学》2004年第5期105-109,共5页Scientia Silvae Sinicae

基　　金：国家重点基础研究发展规划 (G19990 160 0 5 ) ;国家转基因植物研究与产业化专项 (J0 0 B 0 0 1 0 1;J2 0 0 2 B 0 0 3 )。

摘　　要：利用PCR分析技术 ,分析了田间试验已达 7a的转Bt基因欧洲黑杨的基因稳定性 ,并对其林地和非转基因林地的土壤微生物进行了类群数量分析。PCR分析表明Bt基因仍然存在于转基因植株中 ,转基因植株与对照杂交后代Bt基因分离呈 1∶1比例 ,符合孟德尔遗传分离规律 ,表明基因仍稳定存在。计数结果表明转基因林地中转基因植株与非转基因植株间根系土壤 3大类微生物 (细菌、放线菌和霉菌 )数量无显著差异 ,转基因林地与非转基因林地 (邻近杨树林地和健杨林地 )间的土壤 3大类微生物 (细菌、放线菌和霉菌 )数量无显著差异 ,说明转基因欧洲黑杨对土壤微生物系统尚没有明显的影响。It is more than seven years since transgenic Populus nigra carried Bacillus thuringiensis (Bt) gene has been tested in the field. By PCR technology, the gene stability of transgenic P. nigra with Bt gene was analyzed, and the soil microorganism in the transgenic plantation and non transgenic plantation was quantitatively assayed for group. According to PCR analysis, Bt gene was still in the transgenic P. nigra trees, and Mendelian segregation of Bt gene was observed in the progeny of transgenic P. nigra (clone 153) crossed with control (CK 3). In order to evaluate the effects of transgenic forest trees on the environment biosafety, we surveyed three groups of microorganisms (bacterium sum, actinomyces and mould) in the soil around the roots of both non and transgenic trees, and of both non and transgenic plantations. Analyzing using ANOVA, we have found no significant difference in terms of the microorganism number among both the individual trees and the plantations. It indicates that there are still no significant negative effects of transgenic poplar on the soil microorganism system.

关 键 词：欧洲黑杨 转基因植株 基因稳定性 土壤微生物 PCR 生物安全 外源Bt基因 

分 类 号：S792.11[农业科学—林木遗传育种]