GNA基因遗传转化甘蔗研究  

A Study on Genetic Transformation of GNA Gene in Sugarcane

作　　者：陈平华[1] 林美娟[1] 薛志平[1] 陈如凯[1] 

机构地区：[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002

出　　处：《江西农业大学学报》2004年第5期740-743,748,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家863计划(2001AA241191;2002AA241031);福建省青年科技人才创新项目(2001J044);948项目(2003-Q06)

摘　　要：虫害常常给甘蔗生产造成重大损失。GNA对蚜虫、飞虱、叶蝉等刺吸式害虫和某些咀嚼式害虫如蛴螬等有极高的毒性。对引进的GNA基因进行鉴定,并利用基因枪将GNA基因转化甘蔗愈伤组织,经筛选、分化,获得了一批抗性再生植株,抽样检测,获得了4株PCR阳性植株,为培育优良抗虫甘蔗新品种创造了条件。Insect pests always cause significant yield losses to sugarcane.It has been proved that snowdrop lectin (Galanthus nivalis agglutinim, GNA) is toxic to aphids,white flies,leafhoppers etc which belong to sap sucking pests and some chewing pests such as grubs. The GNA gene was identified and transformed into calli of sugarcane via particle bombardment in this experiment. Hundreds of hygromycin-resistant sugarcane seedlings were obtained and four seedlings were PCR-positive in a sample which shows a delighted prospect for the selection of pest-resistant varieties.

关 键 词：GNA 基因枪 甘蔗 转基因 抗虫基因 愈伤组织 

分 类 号：S566.1[农业科学—作物学]