SDS-聚丙烯酰胺凝胶电泳法测定蛇毒抗瘤蛋白的相对分子质量  

Molecular weight determination of atranticance protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

作　　者：陈亚飞[1] 孙宇 高丰衣[1] 周立娜[1] 徐康森[1] 

机构地区：[1]中国药品生物制品检定所生化药品与基因工程药物室,北京100050 [2]北京市医药器械学校,北京100012

出　　处：《中国生化药物杂志》2004年第5期300-303,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的建立改进的低分子SDS 聚丙烯酰胺凝胶电泳法测定蛇毒抗瘤蛋白的相对分子质量 (Mr)。方法考察不同因素对电泳结果的影响 ,对Mr 标准进行直线回归分析 ,确定蛇毒抗瘤蛋白中组分的Mr。结果测得各条带的Mr 分别为 :2 35 0 0、15 4 0 0、1340 0、1130 0、930 0、80 0 0。结论此法供试品处理方便 ,用量少 ,在含 6mol/L尿素的分离胶 (交联度为 16 .5 % ,丙烯酰胺总浓度为 6 % )中能同时显示中、低Mr 的条带 ,是一种测定中、低Mr 的较好方法。PurposeTo establish a modified electrophoresis method for the analysis of peptides with low molecular weights in Atranticance.MethodsInvestigating the effects of different factors. The linear regression analysis was made using molecular markers and the molecular weights of the components in Atranticance were determined.ResultsA method was established. The relative molecular weights of the components in the sample are 23 500,15 400,13 400,11 300,9 300,8 000 respectively.ConclusionSeparating gel with 6 mol/L urea at higher acrylamide concentration (16.5% T,6% C) is useful for identification of middle and low molecular peptides and is a well-established method for the analysis of middle and low molecular peptides.

关 键 词：蛇毒抗瘤蛋白 SDS-聚丙烯酰胺凝胶电泳 相对分子质量 

分 类 号：R979.1[医药卫生—药品]