慢性吸烟大鼠气道平滑肌大电导的钙激活钾通道和Kv1.5表达的变化(英文)  

Effect of chronic cigarette smoking on large-conductance calciumactivated potassium channel and Kv1.5 expression in bronchial smooth muscle cells of rats

作　　者：叶红[1] 马万里[2] 杨木兰[3] 刘声远[1] 王迪浔[1] 

机构地区：[1]华中科技大学同济医学院病理生理系卫生部呼吸系疾病重点实验室 [2]华中科技大学同济医学院附属协和医院呼吸内科 [3]华中科技大学同济医学院病理系,武汉430030

出　　处：《生理学报》2004年第5期573-578,共6页Acta Physiologica Sinica

基　　金：This work was supported by the National Natural Science Foundation of China (No.39770309).

摘　　要：复制大鼠的慢性吸烟模型,采用气道反应性的测定、HE 染色、免疫组织化学染色、原位杂交和免疫印迹实验等方法,观察吸烟对大鼠支气管平滑肌大电导的钙激活的钾通道(BKca)和电压依赖性延迟整流钾通道Kv1.5 蛋白和mRNA表达的影响,以阐明吸烟引起的气道高反应性发病机制中钾通道表达变化的作用。结果显示:(1)慢性吸烟可降低大鼠大气道和小气道BKca 和 Kv1.5 蛋白和 mRNA 表达;(2)大气道 BKca 的降低程度大于 Kv1.5, 小气道 BKca 和 Kv1.5 的降低程度无明显差异;(3)吸烟对全肺组织BKca 和Kv1.5 的蛋白表达无明显影响。上述结果提示, 慢性吸烟可下调大鼠气道平滑肌钾通道BKca 和Kv1.5的表达水平, 是导致气道高反应的机制之一。To investigate the role of potassium channels in the pathogenesis of airway hyperresponsiveness induced by cigarette smoking, the alteration in expression of large–conductance calcium-activated potassium channel (BKca) and voltage-dependent delayed rectifier potassium channel (Kv1.5) in bronchial smooth muscle cells were investigated in chronic cigarette smoking rats. Airway responsiveness was determined, hematoxylin and eosin staining, immuno-histochemistry, in-situ hybridization and western blot techniques were used. The results showed: (1) Chronic cigarette smoking down-regulated the protein synthesis and mRNA expression of BKca and Kv1.5 in bronchial and bronchiolar smooth muscles. (2) BKca decreased more markedly than Kv1.5 in bronchi, but there was no difference between them in bronchioli. (3) No changes in the expression of these two potassium channel proteins were found in extracted cell membrane protein from lung tissue. The results suggest that chronic cigarette smoking can down-regulate the levels of BKca and Kv1.5 in rat bronchial smooth muscle cells in vivo, which might contribute to the mechanism of airway hyperresponsiveness induced by cigarette smoking.

关 键 词：吸烟 支气管平滑肌 钾通道 

分 类 号：R363[医药卫生—病理学]