pYLTAC7载体在水稻品种农垦58S转化中应用的研究  

Study on the Utility of pYLTAC7 Vector in Transformation of Nongken58S (Oryza sativa L. ssp) through Agrobacterium-mediated Method

作　　者：李亚芳[1] 林拥军[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2004年第3期281-284,共4页Journal of Huazhong Agricultural University

基　　金：国家"八六三"高科技计划基金项目 (Z16 0 2 0 2 )资助

摘　　要：利用TAC(transformation competentartificialchromosome)载体 pYLTAC7,将明恢 6 3的 35kb基因组片段通过农杆菌介导的方法导入水稻品种农垦 5 8S中 ,对 pYLTAC7载体直接用于水稻遗传转化的可行性进行了探索性研究。本试验中 ,pYLTAC7载体转化效率为 10 .1% ,转基因植株R0 代PCR及Southern分析表明 ,外源片段已稳定整合到基因组中 ,从而证明pYLTAC7载体可以在水稻遗传转化中应用。By use of TAC vector(transformation-competent artificial chromosome) pYLTAC7, 35 kb genomic DNA of Minghui 63 was transformed into Nongken 58S through Agrobacterium-mediated method, through which an attempt was made on the availability of pYLTAC7 Vector in transformation of monocotyledonous species rice. Results showed: The transformation efficiency was 10.1%. The foreign fragment was integrated into the rice genome stably by PCR and Southern analysis of transformants in the R 0 generation. So, this study provided evidence that pYLTAC7 vector can be used in rice transformation.

关 键 词：pYLTAC7载体 遗传转化 水稻 农垦58S 

分 类 号：S511.310.353[农业科学—作物学]