HPLC法测定止喘灵注射液中东莨菪碱的含量  

Determination the Contents of Scopolamine in Zhichuanling Injection by HPLC

作　　者：刘建华[1] 杨德柱 

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161042 [2]齐齐哈尔市药品检验所,黑龙江齐齐哈尔161006

出　　处：《中国中医药信息杂志》2004年第7期609-610,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的采用高效液相色谱法测定止喘灵注射液中东茛菪碱的含量。方法采用Spherisorb-C18柱(4.6mm×200mm);柱温25℃;流动相:甲醇-水(78∶22),含0.005%十二烷基硫酸钠;流速1.0mL/min;检测波长210nm。结果线性范围为0.6～4.2μg,回归方程为:Y=173305X+2108,r=0.9978,平均回收率为98.94%,RSD=1.03(n=6)。结论此方法准确、可靠,可用于该制剂中东茛菪碱的含量测定。Objective Determination the contents of scopolamine in Zhichuanling Injection by HPLC. Method Spherisorb-C18 column. Column temperature was 25 ℃ . The mobile phase was methanol-water-sodium lauryl sulfate (78∶22∶0.005). The UV detection wavelength was 210 nm. Result A good linearity was obtained in the range of 0.6～4.2 μg. Regression:Y=173305X +2108, r =0.9978. The average recovery was 98.94%, RSD=1.03% (n =6). Conclusion The method being good for determining scopolamine in Zhichuanling Injection was accurate and reliable.

关 键 词：高效液相色谱法 止喘灵注射液 东莨菪碱 含量测定 

分 类 号：R284.1[医药卫生—中药学]