愈障汤对慢性再生障碍性贫血患者骨髓CD_(34)细胞体外增殖的影响  

Effect of Yuzhang Decoction on In Vitro Proliferation of Bone Marrow CD_(34) Cells in the Patient of Aplastic Anemia

作　　者：王树庆[1] 赵典刚[1] 张圣明[2] 李义清[1] 刘捷[1] 李建华[1] 

机构地区：[1]潍坊医学院附属医院中医科 [2]潍坊医学院组织胚胎教研室

出　　处：《中医杂志》2004年第6期432-433,共2页Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局科研基金(00-01LP37);山东省科技厅科研基金(971226209)

摘　　要：目的:观察愈障汤对慢性再生障碍性贫血(CAA)患者骨髓CD34细胞体外增殖的影响,探讨其作用机制.方法:采用单克隆抗体-免疫磁株分离系统(MACS)分离纯化CAA患者骨髓CD34细胞,并进行细胞培养.采用氚标-胸腺嘧啶核苷(3H-TdR)掺入法和液体闪烁技术,检测愈障汤对骨髓CD34细胞的增殖作用.结果:愈障汤组骨髓CD34细胞呈浓度依赖性增殖.其放射活性(cpm)与其它各浓度组比较差异有显著性(P＜0.05或P＜0.01).结论:愈障汤在体外有促进CAA患者骨髓CD34细胞增殖的作用.其治疗CAA的机制可能是通过增殖与抑凋亡的双向调节作用来实现的.Objective: To observe effects of Yuzhang Decoction on in vitro proliferation of bone marrow CD_(34) cells in the patient of aplastic anemia (CAA) and its mechanism. Methods: To adopt monoclone antibody-immune magnetic beads separation system (MACS) to separate and purify CD_(34) cells in bone marrow of the patient of CAA, and make cell culture, applying ~3H-TdR incorporation to detect effect of Yuzhang Decoction on proliferation of CD_(34) cells of bone marrow. Results: CD_(34) cells of bone marrow showed concentration-dependent proliferation in the Yuzhang Decoction group with significantly differences (P<0. 05 or 0. 01). Conclusion: Yuzhang Decoction can improve in vitro proliferation of CD_(34) cells of bone marrow of the patient of CAA. Yuzhang Decoction curing CAA is carried out possibly through promoting proliferation of CD_(34) cells and inhibiting apoptosis of CD_(34) cells.

关 键 词：愈障汤 慢性再生障碍性贫血 骨髓 CD34 细胞增殖 中医药疗法 

分 类 号：R259[医药卫生—中西医结合]