^(51)Cr释放法检测赖氨酸锗对NK细胞活性的作用  

The efiFect of lysine-germanium on natural killer activity by ^(51)Cr release assay

作　　者：陈红专[1] 孙琛[1] 俞霭瑶[1] 金正均[1] 洪金秧 朱炳法[2] 

机构地区：[1]上海第二医科大学基础医学院,上海200025 [2]上海市免疫学研究所

出　　处：《核技术》1993年第2期99-102,共4页Nuclear Techniques

摘　　要：采用^(51)Cr释放法,以K_(562)瘤株为靶细胞,观察赖氨酸锗(Ge_(401))对正常人外周血NK细胞活性(NK-A)的作用。结果表明:Ge_(401)在50、100、200μg/ml三种浓度与效应细胞预处理18h后,NK-A(％)从对照组24.30分别提高为32.52(p<0.05),39.70(p<0.01),43.42(p<0.001),呈明显的剂量依赖性。同时,Ge_(401)在100、200μg/ml两种浓度时,对^(51)Cr标记的K_(562)靶细胞的^(51)Cr释放率无明显直接影响。提示Ge_(401)对K_(562)靶细胞无直接细胞毒作用,其促进NK-A的作用部位在于效应细胞。The effect of lysine- germanium (Ge401), a novel organogermanium on the natural killer activity (NK- A) in human peripheral blood was studied using 51Cr labelled K562 cells as target cells. Ge401 (50, 100 and 200 μg/ml) could augment NK- A(%) from 24.30 ±6.05 of the control group to 32.52±6.07 (p<0.05), 39.70±7.38 (p<0.01) and 43.42±7.71 (p<0.001) respectively, showing obvious dose- dependence. However, there is no direct influence of Ge401 (100 and 200 μg/ml) on 51Cr release rate of Ks562 leakemia cells.

关 键 词：赖氨酸 锗 杀伤细胞 铬51 释放法 

分 类 号：R817.1[医药卫生—影像医学与核医学]