^(60)Co γ射线辐照对小麦愈伤组织超微结构的影响  

INFLUENCE OF CALLUS IRRADIATION WITH ^(60)CoγRAYS ON THE CELL ULTRASTRUCTURE OF WHEAT

作　　者：胡天赐[1] 高明尉[1] 梁竹青[1] 

机构地区：[1]浙江农业大学原子核农业研究所,杭州310029

出　　处：《核农学报》1993年第1期16-20,共5页Journal of Nuclear Agricultural Sciences

摘　　要：用^(60)Coγ射线不同照射量辐照旺盛生长的小麦愈伤组织,并于辐照后3、6、12天取材固定进行电镜观察。结果发现,未经辐照的愈伤组织细胞超微结构正常。经500Rγ射线辐照后,细胞结构有轻度损伤,随之出现旺盛的修复活动,线粒体数量明显增加,内嵴结构完善,内质网发达;辐照后12天细胞结构基本恢复正常。经5000R辐照后细胞严重受损,结构逐趋模糊,细胞器逐步消解,最后细胞死亡。结合辐照后愈伤组织的生长状况以及再生株染色体变异频率等,认为对小麦愈伤组织进行辐射处理时照射量以500～1000R为宜。The calli of immature embryo derived from wheat were irradiated with 60Co γ-rays of differ-.ent dosage and were fixed at different time after irradiation.The cell ultrastructure observed with electron microscope indicated that the non-irradiated calli had normal cell ultrastructure while the cell of calli irradiated with 500Rγ-rays suffered slight damages in three days after irradiation and repairing activities,including the increase of mitochondrion,the completion of crista structure and the development of endoplasmic reticulum were seen.On the 12th day after irradiation,the cell ultrastructure retuned to normal state.When calli irradiated with 5000R γ-rays,the cell ultra-structure were damaged seriously and became unclear,cell organs disintegrated,which finally leading to cell death.Considering the calli growth situation and the frequency of chromosomal variation in regenerated plants after irradiation,we suggest that 500 to 1000Rγ-rays be appropriate doses for callus irradiation in wheat.

关 键 词：小麦 愈伤组织 钴60 Γ射线 

分 类 号：S512.103.5[农业科学—作物学]