慢性鼻-鼻窦炎患者嗅觉功能和嗅觉标记蛋白的研究  

Olfactory function and olfactory marker protein in patients with chronic sinusitis

作　　者：高海燕[1] 诸小侬[2] 彭诗东[1] 张呼和[1] 范尔钟[2] 王鸿[2] 韩德民[2] 

机构地区：[1]内蒙古医学院第一附属医院耳鼻咽喉科,呼和浩特010050 [2]北京市耳鼻咽喉科研究所

出　　处：《中华耳鼻咽喉科杂志》2004年第8期493-495,共3页Chinese Journal of Otorhinolaryngology

摘　　要：目的 探讨慢性鼻-鼻窦炎患者嗅觉功能障碍的发病机制和嗅觉标记蛋白(olfactionmarker protein,OMP)在嗅觉黏膜中的表达及意义。方法 对55例慢性鼻-鼻窦炎和11例对照组患者用T＆T嗅觉计定量检查法进行嗅觉功能主观检测,并应用免疫组化技术检测其嗅黏膜嗅觉标记蛋白的表达和分布。结果 慢性鼻-鼻窦炎患者主诉有嗅觉障碍者为50.9％(28/55),经主观嗅觉功能检测嗅觉障碍者为85.5％(47/55);对照组患者主诉有嗅觉障碍者为9.09％(1/11),经主观嗅觉功能检测嗅觉障碍者为18.2％(2/11),差异有显著性(x2=9.86,P<0.01)。对两组嗅黏膜进行OMP免疫组化染色显示:OMP存在于嗅黏膜的嗅细胞和微绒毛细胞中,OMP染色细胞数随嗅觉障碍程度加重而逐渐减少或消失。结论 慢性鼻-鼻窦炎患者嗅觉障碍的主要原因是嗅黏膜中嗅细胞的减少、萎缩和退化所导致的对嗅素的不能察觉和认识。Objective To study the pathogenesis of dysosphresia in patients with chronic sinusitis, to discuss the expression and significance of olfaction marker protein in olfactory mucosa. Methods There were 55 cases with chronic sinusitis in the experimental group, and 11 cases in the control. All of them were first tested by subjective method, and then the olfactory marker protein in olfactory mucosa was examined by the method of immunohistochemistry. Tissue sections were observed under optical microscope. Results The ratio of dysosphresia in patients with chronic sinusitis was 50. 9% (28/55 ) , while by subjective olfactory test, it was 85.5% (47/55). The ratio of dysosphresia in control patients was 9.09% (1/11), by subjective olfactory test, it was 18. 2% (2/11). Olfactory marker protein was identified in the olfactory cells and microrillar cells by histochemical and immunohistochemical technique ( S-P method). Conclusions The main cause of dysosphresia in patients with chronic sinusitis is the decrease, atrophy and degeneration of the olfactory cells, which lead to the failure of olfactus perception and recognition.

关 键 词：慢性鼻炎 慢性鼻窦炎 嗅觉功能 嗅觉标记蛋白 嗅觉障碍 

分 类 号：R765[医药卫生—耳鼻咽喉科]