广西壮族、仫佬族和越南中部京族儿童幽门螺杆菌感染与HLA-DQA1相关性的研究  

Association of HLA-DQA1 loci with Helicobacter pylori infection in children of Guangxi Zhuang, Mulam nationalities, China and central Jing nationality, Vietnam

作　　者：王琳琳[1] 陈亭平[2] 林伟雄[3] 姜海行[4] 单庆文[1] 谢湘芝[3] 梁淡湄[1] 

机构地区：[1]广西医科大学第一附属医院儿科,南宁530021 [2]广西医科大学 [3]广西医科大学医学实验中心 [4]广西医科大学第一附属医院消化内科

出　　处：《中华儿科杂志》2004年第8期577-580,共4页Chinese Journal of Pediatrics

基　　金：广西自然科学基金资助(0135026)

摘　　要：目的 探讨广西罗城县壮族、仫佬族儿童与越南中部京族儿童间幽门螺杆菌(helicobacter pylori,Hp)感染与人类白细胞抗原DQAl(HLA-DQAl)等位基因位点的关系。方法(1)广西罗城县壮族儿童54名,仫佬族儿童76名,平均年龄11岁;京族儿童109名,平均年龄13岁。采用间接酶联免疫吸附法(ELISA)检测血清抗Hp—IgG抗体,采用免疫印迹法检测血清中Hp空泡毒素、毒素相关蛋白及尿素酶抗体;(2)采用聚合酶链反应-序列特异引物(PCR-SSP)方法对3个民族儿童的HLA—DQAl等位基因进行检测,并采用疾病关联分析法进行HLA-DQAl等位基因分布与Hp感染检测结果相关性分析。结果 (1)越南中部京族儿童Hp阳性率(39％)明显低于广西壮族及仫佬族儿童(65％、58％,P<0.01);(2)广西壮族、仫佬族儿童及越南京族儿童HLA-DQAl*0104等位基因频率在Hp阳性儿童人群均高于Hp阴性儿童(P<0.01),相对风险度分别为5.57、4.18及3.45。结论(1)广西壮族、仫佬族儿童的Hp感染率高于越南中部京族儿童;(2)3个民族的Hp阳性和阴性儿童在HLA—DQAl*0104等位基因上存在差异,HLA—DQAl*0104等位基因可能是Hp感染的易感基因。Objective To compare the prevalence of Helicobacter pylori (Hp) infection in children of Zhuang and Mulan ethnic groups, Guangxi Luocheng county, China and in children of Jing ethnic group, Central Vietnam and to analyze the association of HLA-DQAl alleles of these ethnic groups'children with Hp infection. Methods Serodiagnosis by determining Hp antibody with ELISA and determination of serum CagA, VacA and urease antibodies by immunoblotting were performed for 54 Zhuang, 76 Mulan and 109 Jing children. Polymerase chain reaction-single strand polymorphism (PCR-SSP) technique was applied to determine the polymorphism of the HLA-DQAl locus of these children and then the association of HLA-DQAl alleles of these minority children with Hp infection was analyzed by SAS software. Results The prevalence of Hp infection were 39% in Vietnamese Jing nationality, which was significantly lower than that in children of Guangxi Luocheng county (65% in Zhuang nationality and 58% in Mulan nationality) (P < 0. 01). The distribution of HLA-DQAl locus was not significantly different among the 3 groups. The frequency of HLA-DQAl * 0104 allele was significantly higher in children with Hp infection than in children without Hp infection in each of the 3 groups (P < 0. 01). Conclusion The results indicated that the prevalence of Hp infection in Zhuang and Mulan minority ethnic groups in Guangxi, China was higher than that in Vietnamese Jing ethnic group children. HLA-DQAl * 0104 allele may be associated with susceptibility to Hp infection.

关 键 词：广西 壮族 仫佬族 越南中部 京族 儿童 幽门螺杆菌感染 HLA-DQA1 相关性 

分 类 号：R725.7[医药卫生—儿科]