组织工程补片修补犬腹主动脉的实验研究  

Study on tissue engineered patch replacing segment of the canine abdominal aorta

作　　者：高峰[1,2] 胡平[2] 周建业[1] 高峰[1,2] 孟亮[1] 胡盛寿 

机构地区：[1]中国医学科学院中国协和医科大学心血管疾病研究所阜外心血管病医院瓣膜研究室 [2]清华大学化工系高分子材料和化工研究所

出　　处：《中华胸心血管外科杂志》2004年第4期230-233,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家高科技研究发展计划 ( 863计划 )项目资助( 2 0 0 1AA2 160 71);国家杰出青年科学基金资助 ( 3 0 12 5 0 3 9)

摘　　要：目的　评估新共聚多聚体生物可降解材料 -p(3HB co 3HH) PU制成的组织工程补片植入到主动脉系统的可行性。方法　将杂种犬分为实验组 (植入组织工程补片 ,6只 )和对照组 (植入空白补片 ,4只 )两组。术后分批处死实验犬 ,取出补片 ,分别做病理检查、扫描电镜检查和生化检测。结果　肉眼所见 ,组织工程补片内侧面光滑 ,有内膜样组织形成 ,没有血栓 ;而空白对照组补片内侧面粗糙 ,有血栓样组织形成 ;两组补片均无瘤样扩张。HE染色显示组织工程补片在植入 4 8周后 ,其显微结构和细胞外基质的排列与自体主动脉类似 ;ET +VG染色显示组织工程补片中有弹力纤维和胶原纤维存在 ,形态与自体主动脉相似 ;而对照组中弹力纤维和胶原纤维含量少 ,排列松散。免疫组化染色显示在组织工程补片的内侧面 ,有连续的VIII因子染色阳性的单层内皮细胞存在 ,扫描电镜证实为内皮细胞 ;生化检测显示组织工程补片中胶原蛋白和弹性蛋白的含量均高于空白对照组。结论　在生物可降解的p(3HB co 3HH) PU补片上种植自体肌成纤维细胞和内皮细胞 ,植入到犬腹主动脉 ,可形成与自体主动脉类似的组织工程动脉。Ovbective To evaluate the feasibility of a tissue engineered patch constructed by a new copolymer of p(3HB-co-3HH) and polyurethane(PU) as in partial aortic replacement. Methods The autologous cell-polymer scaffold were used to replace 2.0～ 2.5cm abdominal aortic segments in dogs (group TE,n=6). The patches were seeded with 1×10 7 myofibroblasts each day for 4 days,and then 1×10 7 endothelial cells were seeded onto the scaffold. After 48 hours' incubation,the cell-polymer scaffolds were implanted to replace a segment of canine abdominal. In the control group (n=4),aortic segments were replaced with acellular polymer patches. No anticoagulation agent was used. At 2nd,4th,8th,12th,24th,and 48th weeks after operation,the seeded group animals were killed. The control group animals were killed at 4th,12th,24th,and 48th weeks. Explanted patches were examined histologically with scanning electron microscopy,and biochemically. Results In the group of TE patches,the tissue-engineered patches were covered with endothelium-like tissue macroscopically,and there was no thrombus formation on any of the specimens. Histological staining showed uniform layered tissue with endothelium and laminated fibrous tissue with collagen as predominant extracellular matrix. A confluent smooth surface was observed by scanning electron microscopy. An increase in the content of collagen and elastin was observed,and at 48th weeks after operation and their contents equaled to the level of native aorta. There was no endothelium formation in the acellular control,the collagen and elastin content were also smaller than that of the TE groups. Conclusion The autologous aortic grafts with biological characteristics resembling the native aorta can be created by using TE approach.

关 键 词：组织工程 补片 修补 犬 腹主动脉 实验 人工血管 

分 类 号：R654.3[医药卫生—外科学]