焦炉作业工人多环芳烃暴露与外周血淋巴细胞DNA损伤的关系  

Relationship of polycyclic aromatic hydrocarbon exposure with peripheral blood lymphocyte DNA damage in coke oven workers

作　　者：冷曙光[1] 郑玉新[1] 牛勇[1] 谷也平[1] 张文众[1] 戴宇飞[1] 王雅文[1] 李晓华[1] 潘祖飞[2] 肖军[2] 王忠旭[3] 李涛[1] 何凤生[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所院士实验室,北京100050 [2]辽宁省本溪钢铁集团有限责任公司劳动卫生研究所 [3]辽宁省鞍山钢铁集团公司劳动卫生研究所

出　　处：《中华劳动卫生职业病杂志》2004年第4期250-253,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助 ( 3 9970 63 3 );国家重点基础研究规划项目资助项目 ( 2 0 0 2CB5 12 90 3 )

摘　　要：目的　探讨焦炉作业工人多环芳烃 (polycyclicaromatichydrocarbon ,PAHs)暴露与其外周血淋巴细胞DNA损伤的关系。方法　选取 2 35名焦炉作业工人和 30名非职业PAHs暴露者 ,使用碱性单细胞凝胶电泳技术评价外周血淋巴细胞DNA损伤 ,采用高效液相色谱法测定工作周末班后 6h尿中 1 羟基芘水平 ,收集个人职业暴露、年龄、性别、吸烟和饮酒等信息。结果　焦炉工外周血淋巴细胞的Olive尾矩高于对照组 [数值分别为 2 .4 7(0 .2 2～ 4 6 .6 8)、0 .94 (0 .4 2～ 4 .2 1) ],差异有显著性 (P <0 .0 1) ,且与尿中 1 羟基芘水平呈相关性 (Spearman偏相关系数 =0 .2 2 ,P <0 .0 1)。以非暴露者Olive尾矩的上 5分位数 1.9作为判断个体是否为DNA损伤阳性的界值 ,焦炉工发生外周血淋巴细胞DNA损伤阳性的OR =5 .38,95 %CI=2 .0 7～ 14 .0 8,且随外暴露等级或内剂量水平的增高而呈增加的趋势。结论　焦炉工PAHs暴露水平与外周血淋巴细胞DNA损伤呈良好的剂量 -效应和剂量 -反应关系。Objective To investigate the relationship between lymphocyte DNA damage and polycyclic aromatic hydrocarbons(PAHs) exposure in coke oven workers. Methods Two hundred and thirty-five coke oven workers and 30 controls were selected in this study.Alkaline single-cell gel electrophoresis was used to evaluate the lymphocyte DNA damage,HPLC was employed to measure 1-hydroxypyrene levels in spot urine samples which were obtained at the end of a workweek(4 days of 8 hours/day) and personal information including occupational exposure,age,sex,smoking and drinking status was collected by the questionnaire. Results The lymphocyte DNA damage level expressed as olive moment in coke oven workers was significantly higher than that of controls [2.47(0.22～46.68) vs 0.94(0.42～4.21),P<0.01],and correlation between urinary 1-hydroxypyrene concentrations and olive moment was found(Spearman Partial correlation coefficient= 0.22,P<0.01) in coke oven workers.The 1.9 of olive moment value was used as the limit to determine whether the subject DNA damage was positive.The coke oven workers had significantly higher risk in DNA damage(adjusted OR=5.38,95% CI=2.07～14.08) than did controls,and dose-response relationships were also found between external exposure(exposure category) or internal doses(urinary 1-hydroxypyrene) and DNA damage. Conclusion There are dose-effect and dose-response relationships between PAHs exposure and lymphocyte DNA damage in coke oven workers.

关 键 词：焦炉作业工人 多环芳烃 外周血 淋巴细胞 DNA损伤 PAHS 多环碳氢化合物 

分 类 号：R131[医药卫生—劳动卫生]