小鼠不同周龄与骨密度的关系  

Age-related changes in bone mineral density and cross-sectional area in mice

作　　者：严雪铭[1] 张振德[1] 

机构地区：[1]中国科学院上海药物研究所,上海201203

出　　处：《中国骨质疏松杂志》2004年第3期288-289,283,共3页Chinese Journal of Osteoporosis

摘　　要：目的　采用pQCT设备 ,以整体小鼠胫骨为检测对象 ,了解不同周龄骨密度含量以及不同组分面积 ,为进行小鼠骨代谢研究提供参考。方法　 4～ 12周龄昆明种小鼠 30只 ,雌性 ,每 6只为 1组。用戊巴比妥钠麻醉后 ,固定于检测台上 ,以右后肢为被测对象 ,采用德国产动物用pOCT设备 ,分别对胫骨近端骨骺线 1 4mm和 4 0mm处进行测定。结果　小鼠 4～ 12周龄 ,体重增长很快 ,周龄与体重的相关性r=0 96 3,P <0 0 0 1;此阶段胫骨总骨密度、皮质骨密度随着周龄的增长而增加 ,呈非常显著的正相关 ;周龄与骨小梁的骨密度无明显相关性 ;8周龄时胫骨小梁面积最大。结论　pQCT能灵敏、精确的测定整体小鼠胫骨 ,有效地区分各个不同组分 ,可以动态的观察骨骼的变化 ,以 8～ 10周龄的小鼠适宜造模型。Objective Age-relatde changes in bone mineral density(BMD),cross-sectional area of the tibia in vivo were measured by peripheral quantitative computed tomography(pQCT),and the correlation was analyzed,providing the reference for the resarch on bone metabolism in mice. Methods Thirty female mice were divided into age groups of 4,6,8,10 and 12 weeks, 6 each. The mice were fixed with holder under anesthesia.BMD and cross-sectional area were measured by pQCT(Stratec,XCT,Research SA)at 1.4 and 4.0 mm from the proximal epiphysis of tibia. Results The body weight of female mice increased quickly during 4-12 weeks of age,and showed age dependemcy with significant correlation (r=0.963,P<0.001);the total and cortical BMD have increased at 4-12 weeks as well as corss-sectional area,and they were correlated significantly.But trabecular BMD increased slowly without correlation during the period,and the maximal trabecular area appeared at 8 weeks of age. Conclusion The pQCT precisely defermines various regions of mouse tibia in vivo. Therefore, measurement in vivo is useful for the monitoring of skeletal changes in mice.Mice at the ages of 8-10 weeks are suitable for development of osteoporosis models.

关 键 词：小鼠 骨密度 胫骨近端 骨小梁 QCT 相关性 皮质骨 造模 戊巴比妥钠 骨代谢 

分 类 号：R681[医药卫生—骨科学] R683[医药卫生—外科学]