不同剂量NO供体防治骨质疏松大鼠的实验研究  

Effects of different doses of nitric oxide donor on prevention of rat osteoporosis

作　　者：镐英杰[1] 陈凤苞[2] 唐煜[3] 裴福兴[1] 

机构地区：[1]四川大学华西医院骨科,成都610041 [2]郑州大学第一附属医院骨科 [3]郑州大学医学院

出　　处：《中国骨质疏松杂志》2004年第3期290-292,301,共4页Chinese Journal of Osteoporosis

摘　　要：目的　探讨不同剂量的NO供体 (硝酸甘油 )对去卵巢大鼠骨质疏松的防治作用。方法　 4月龄雌性大鼠随机分为假手术组 (SO组 )和骨质疏松模型组 ,其中骨质疏松模型组待造模成功 1周后 ,再随机分为骨质疏松组 (OVX组 )和低剂量硝酸甘油组 (lowdosenitroglycerin ,LNG组 )、中剂量硝酸甘油组 (middledosenitroglycerin ,MNG组 )以及高剂量硝酸甘油组 (highdosenitroglycerin ,HNG组 )。于灌胃给药 12周后测量各组大鼠全身骨密度 ,右侧股骨干重、灰重及灰分钙含量 ,并测量血清生化指标等参数。结果　OVX组大鼠相比SO组股骨干重、灰重、灰分钙含量及血清NO、Ca含量下降 ,血清P含量升高 ;LNG和MNG组大鼠密度升高后 ,与OVX组相比除血清P水平下降其余检测指标均显著升高 ;HNG组大鼠与OVX组相比血清NO水平显著升高 ,其余指标经统计学处理差异无显著性。结论低、中剂量硝酸甘油具有明显防治去卵巢大鼠骨质疏松作用而高剂量硝酸甘油不能阻止去卵巢大鼠骨质退化 ,骨量丢失。Objective To study the preventive effects of nitric oxide donor (nitroglycerin) on rat osteoporosis. Methods Four-month-old female SD rats were randomized into sham-operated (SO) group and ovariectomized(OVX) group. One week later, the OVX rats were further randomized into OVX-induced osteoporosis (OVX) group, low dose nitroglycerin(LGN) group, middle dose nitroglycerin (MNG) group, and high dose nitroglycerin (HNG) group. After the treatment, the whole body bone mineral density (BMD) and bone metabolism-associated biochemical markers were determined. The dry weight, ash weight and calcium content of femoral bone were measured. Results Compared with SO group, the dry weight, ash weight, calcium content of femoral bone, and the serum level of calcium and NO were significantly lower, while the level of serum phosphorus was significantly higher. After BMD in LNG group and MNG group increased, level of serum phosphorus was significantly lower than that in OVX group, while the other markers were significantly higher. All the markers other than serum NO were not significantly different between HNG group and OVX group. Conclusions Low dose nitroglycerin and middle dose nitroglycerin prevent bone loss in OVX rats but hight dose nitroglycerin does not prevent osteoporosis induced by ovariectomy.

关 键 词：大鼠 硝酸甘油 骨质疏松 升高 血清 不同剂量 股骨干 中剂量 高剂量 供体 

分 类 号：R681[医药卫生—骨科学] R285.5[医药卫生—外科学]