佳辐占和广陆矮4号水稻叶片蛋白质的双向电泳比较研究  

Comparative Studies on the Leaf Proteins of Jiafuzhan Rice and Guanluai(4#)Rice by Two-dimensional Electrophonesis

作　　者：林建峰[1] 石艳[1] 陈清西[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2004年第B08期40-43,共4页Journal of Xiamen University：Natural Science

基　　金：厦门大学预研基金;教育部跨世纪人才经费资助

摘　　要：应用双向电泳技术研究比较两种不同品质水稻(佳辐占和广陆矮4号)叶片的蛋白质组分的差异.两种水稻叶片提取的蛋白质的双向电泳图谱斑点清晰,形态规则.采用考马斯亮蓝染色获得约400个斑点,银染图谱约800个斑点.两种水稻的蛋白质图谱考马斯亮蓝染色图谱存在5个显著差异点,银染图谱存在11个显著差异点.Two-dimensional electrophonesis (2D-PAGE) was used to study the different compositions between rice species of Jiafuzhan and Guanluai 4#.The spots in the 2D-PAGE were clear and inerratic. There were about 400 spots and 800 spots detected by Coomassie Brilliant Blue-stained and by silver-stained respectively.There were five different spots between two species of rice in Coomassie Brillian Blue-stained gel and eleven different spots between two species of rice in silver-stained gel.

关 键 词：水稻 蛋白质 双向电泳 品质差异 粮食作物 染色 

分 类 号：S511[农业科学—作物学] Q946[生物学—植物学]