牛黄对三氯乙烯致ICR小鼠DNA损伤的影响  

THE EFFECT OF COW-BEZOAR ON DNA DAMAGE OF ICR MICE DUE TO TCE

作　　者：邰昌松 [1] 黎燕 [2] 杨青 [2] 沈惠麒 [2] 

机构地区：[1]深圳市宝安区疾病预防控制中心,深圳,518101 [2]四川大学华西公共卫生学院劳动卫生教研室

出　　处：《现代预防医学》2004年第4期483-484,486,共3页Modern Preventive Medicine

基　　金：深圳市科技局资助项目 ( 2 0 0 3 0 42 62 )

摘　　要：目的 :研究牛黄 (Cow- bezoar)对三氯乙烯 (TCE)致 ICR小鼠肝肾外周血细胞 DNA损伤的影响。方法 :应用单细胞凝胶电泳 (SCGE)技术和彗星图象分析系统 ,检测三氯乙烯对 ICR小鼠肝肾外周血细胞 DNA损伤作用 ,同时观察2 0～ 2 0 0 μmol/ L 牛黄对三氯乙烯所致 DNA损伤的保护作用。结果 :TCE染毒组肝、肾、血细胞彗星率较阴性对照组增加 ;牛黄各组肝、肾、血细胞尾长、尾 /头 (长 )、尾 /头 (光强 )、尾 DNA%、Oliver尾矩、彗星率减少。结论 :牛黄能抑制三氯乙烯致 ICR小鼠肝肾外周血细胞的 DNA断裂能力。Objective:To study the effect of Cow-bezoar on DNA damage of ICR mice due to TCE.Methods:Single cell gel electrophoresis assay was used to observe DNA damage due to TCE.Different dose of Cow-bezoar was added simultaneously to study the protective effect on DNA damage by TCE.Results:There were dose-response relationship between Cow-bezoar and indexes including extent of tail,tail/head(extent),tail/head(light-intensity),tail% DNA,tail Olive moment,and rate of comet.And inhibition from Cow-bezoar to damage on single strand DNA of TCE in hemocyte,hepatolyte and renal cells was detected.Conclusion:TCE could cause DNA damage of ICR mice and these damages could be inhibited by Cow-bezoar

关 键 词：牛黄 三氯乙烯 ICR 小鼠 DNA损伤 毒理学 

分 类 号：R99[医药卫生—毒理学]