提高二袖套法大鼠原位肝移植成功率的手术技巧  

Surgical Technique of Orthotopic Liver Transplantation Using Two-Cuff Technique and Prevention of Operational Complications in Rats

作　　者：谈燚[1] 黄陈[1] 李宗狂[1] 高涌[1] 刘会春[1] 刘金新[1] 

机构地区：[1]蚌埠医学院附属医院肝胆外科,安徽蚌埠233004

出　　处：《中国普外基础与临床杂志》2004年第5期405-407,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：安徽省教育厅优秀青年科研项目 (2 0 0 2jq14 4)~~

摘　　要：目的　探讨二袖套法大鼠原位肝移植的手术技巧。方法　在Kamada“二袖套法”基础上进行改良。获取供肝前阻断肝门血供 10min ,再灌注 10min ;分别经腹主动脉和门静脉对肝脏进行双重灌注 ;门静脉和肝下下腔静脉用袖套法吻合 ,肝上下腔静脉用缝合法吻合 ,胆总管采用单管内支架胆管端端吻合法。结果　共施行大鼠原位肝移植 12 0次 ,手术成功率为 90 .8%。平均无肝期为 ( 2 1.0± 3.5 )min ,受体总手术时间为 ( 4 6 .0± 4 .5 )min ,1周生存率为 87.2 %。结论　良好的手术野暴露 ,娴熟的显微外科技术 。Objective To investigate the surgical technique of orthotopic liver transplantation using two cuff technique and prevention of operational complications in rats. Methods The model was established with modified cuff technique.Before donor livers were harvested,the portal vein and hepatic artery were interrupted for 10 min,and reflow was initiated for another 10 min.The donor liver was perfused through abdominal aorta and portal vein respectively.The infrahepatic vena vein and portal vein were anastomosed by means of cuff method; the anastomosis of the suprahepatic vena vein was performed with suture method. The anastomosis of the common bile duct was performed with an internal stent. Results One hundred and twenty rats underwent orthotopic liver transplantation using two cuff technique and the successful rate was 90.8%. The average nonhepatic time of recipients was (21.0±3.5) min and the total surgical time was (46.0±4.5) min. The one week survival rate of recipients was 87.2%. Conclusion Good exposure of operative field, sophisticated microsurgical technique and delicate surgical manipulation can benefit decreasing nonhepatic and total surgical time of recipients and increasing the survival rate of recipients.

关 键 词：二袖套法 大鼠 原位肝移植 成功率 手术技巧 

分 类 号：R657.3[医药卫生—外科学]