高效液相色谱法测定山楂中枸橼酸的含量  

Determination of citric acid in Fructus Crataegi by HPLC

作　　者：杨滨[1] 李化[1] 汤树良[1] 陈美兰[1] 

机构地区：[1]中国中医研究院中药研究所,北京100700

出　　处：《中国药学杂志》2004年第9期697-699,共3页Chinese Pharmaceutical Journal

基　　金：国家十五科技攻关计划项目(2001BA701A11-05)

摘　　要：目的建立山楂中枸橼酸的高效液相含量测定方法。方法 高效液相色谱法,Zorbax-sbC18柱,磷酸二氢铵-磷酸缓冲液为流动相,流速1.0 mL·min-1;检测波长为210 nm;柱温25℃。结果枸橼酸回归方程Y=3 510.8+56 992.3X,r=0.999 9,线性范围0.79-15.96μg。生山楂中枸橼酸平均回收率为103.4％,RSD=0.9％;焦山楂中枸橼酸平均回收率为98.8％,RSD=2.8％(n=5)。结论该方法简便易行,可用于山楂饮片中枸橼酸的含量测定。OBJECTIVE: To establish a RP-HPLC method for the determination of citric acid in Fructus Crataegi and Fructus Crataegi Preparatus, and to analyse the content of citric acid in samples collected from different regions. METHODS: A Zorbax sbC18 (250 mm × 4.6 mm, 5 μm) column was used with phosphate buffer solution (pH 3.0) as the mobile phase at a flow rate of 1.0 mL&middotmin-1. The column temperature was 25°C and the UV detection wavelength was 210nm. RESULTS: The calibration curve for citric acid was in good linearity over the range of 0.79-15.96 μg with the regression equation Y = 3 510.8 + 56 992.3 X, r = 0.999 9. The average recoveries of citric acid in Fructus Crataegi and Fructus Crataegi Preparatus were 103.4% and 98.8%, respectively. CONCLUSION: The method is simple, sensitive and reproducible.

关 键 词：山楂饮片 枸橼酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]