醛固酮介导的肾性高血压相关靶器官组织钙调神经磷酸酶活性的变化  

Alteration of tissue calcineurin activity mediated by aldosterone in target organs of renal hypertensive rats

作　　者：陈雯[1] 谢晓华[1] 常连庆[1] 孙愚[1] 李朝晖 卢文宁[1] 李晓玲[1] 刘宁 刘秀华[1] 

机构地区：[1]解放军总医院,北京100853 [2]解放军272医院,天津300020

出　　处：《中国现代医学杂志》2004年第17期58-61,共4页China Journal of Modern Medicine

基　　金：受"十五"军队医药卫生科研基金重点课题资助(02Z010)

摘　　要：目的研究醛固酮介导一肾一夹肾性高血压大鼠脑、肾、脾、肺、主动脉和肝脏组织钙调神经磷酸酶(CaN)活性的变化。方法取20只Wistar 大鼠随机分为3组:假手术组(sham op, n=6),一肾一夹手术组(op,n=7)和螺内酯干预组(spiro,n=7)。以PNPP为底物测定术后4周大鼠各组织器官CaN活性。结果肾性高血压大鼠脾、肾脏、肺、脑CaN活性较假手术组分别上升90%,84%,57%和43%(均P<0.05),螺内酯干预组上述组织器官CaN活性较手术组明显下降(P<0.05)。肾性高血压大鼠组肝脏CaN活性较假手术组下降27%(P<0.05),螺内酯干预组肝细胞CaN活性较手术组有下降趋势,但差异无显著意义。各组大鼠主动脉中CaN活性无明显变化。结论醛固酮对肾性高血压相关靶器官—脑、肾、脾、肺组织细胞内信号分子CaN的激活具有重要作用。Objective: To study the role of aldosterone on the alteration of the tissue calcineurin (CaN) activity in brain,kidney,spleen,lung,aorta and liver of the renal hypertensive rats. Methods: Twenty male rats were divided into 3 groups randomly: sham operated group(sham op group, n =6), 1K1C(one-kidney and one-clip) group (op group, n =7). The rats were treated by the right nephrectomy and chronic partial occlusion of left renal artery for 4 weeks,spironolactone group(spiro group, n =7). CaN activity was measured by using PNPP as substrate. Results: Compared with sham op group rats, the tissue CaN activity in spleen, kidney, lung, brain of op group rats was increased by 90%, 84%, 57% and 43%, respectively (P <0.05). Spironolactone could reverse the increases of CaN activity in these organs and tissues (P <0.05). CaN activity in liver of RHRs was decreased by 27% (P <0.05), which was no significant further decrease in spiro-treated rats (P >0.05). CaN activity in aorta of three groups were no significant differences. Conclusions: Aldosterone might play an important role in activating signal molecule-CaN of related target organs, such as spleen, kidney, lung and brain, in renal hypertensive rats.

关 键 词：高血压 钙调神经磷酸酶 醛固酮 

分 类 号：R541.3[医药卫生—心血管疾病]