添加剂对活性人工皮肤胶原海绵支架材料的影响  

Effect of Additives on Collagen Sponges for Bioactive Artificial Skin

作　　者：杨运[1] 周燕[1] 华平[1] 谭文松[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《功能高分子学报》2004年第3期396-400,共5页Journal of Functional Polymers

基　　金：国家高技术研究发展计划课题(2001AA210641);国家重点基础研究发展规划"组织工程的基本科学问题"项目(G1999054309)

摘　　要：　研究了壳聚糖、硫酸软骨素和肝素对胶原海绵理化性质和生物相容性的影响。结果表明:添加壳聚糖后胶原海绵的孔隙率和吸水率下降,添加硫酸软骨素后上升,而添加肝素后无明显变化。三种添加剂均可减少基质收缩,增强材料的抗降解性能,但种间差异不明显。与纯胶原海绵相比,复合海绵可进一步促进细胞的吸附和增殖,其中添加壳聚糖和肝素的效果相当,优于硫酸软骨素,有望应用于构建组织工程化人工皮肤。The effects of additives, including chitosan, chondroitin sulfate and heparin on the physical-chemical characteristics and biocompatibility of collagen sponges were investigated. The results showed that the porosity and water capacity of collagen sponges decrease by adding chitosan, increase by adding chondroitin sulfate, but no effect by heparin. All the three additives produce similar effect to improve the anti-degradability of collagen sponges and reduce the constriction of scaffolds. The composite sponges has greater accelerate effect on cell growth and proliferation than that of pure collagen sponges. However, the effect of chitosan and heparin on cell growth were found more significant than that of chondroitin sulfate. These results propose that the collagen sponges with these additives are potential for the use in constructing tissue engineered bioartificial skin.

关 键 词：添加剂 人工皮肤 胶原海绵 支架材料 壳聚糖 硫酸软骨素 肝素 

分 类 号：R318.1[医药卫生—生物医学工程]