银杏叶提取物对缺血再灌注大鼠脑内SOD,GSH-Px和MDA的影响  

Effects of Ginkgo biloba extract on SOD, GSH-Px and MDA in brain tissue of rats during cerebral ischemia and reperfusion

作　　者：沈立[1] 郭芳[2] 王永利[2] 沈伏[3] 高松[1] 

机构地区：[1]哈励逊国际和平医院,河北衡水053000 [2]河北医科大学药理教研室,河北石家庄050017 [3]河北省人民医院,河北石家庄050000

出　　处：《中国新药与临床杂志》2004年第10期691-694,共4页Chinese Journal of New Drugs and Clinical Remedies

基　　金：河北省卫生厅 2 0 0 4年科技攻关项目 (0 3192 )

摘　　要：目的 :观察银杏叶提取物注射液对大鼠脑缺血再灌注 1,2 ,3h皮质内超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶 (GSH Px)活性和丙二醛 (MDA)含量的影响。方法 :78只SD大鼠随机分为 13组 :假手术组 ;3个缺血对照组 (缺血 2h分别再灌注 1,2 ,3h组 ) ;3个银杏叶提取物注射液B(金纳多 )治疗组 (缺血 2h分别再灌注 1,2 ,3h组 ) ;6个银杏叶提取物注射液A(杏花雨 )治疗组 (高、低剂量各含缺血 2h分别再灌注 1,2 ,3h组 )。银杏叶提取物注射液B治疗各组分别在再灌注前 1h腹腔注射银杏叶提取物注射液B 5mg·kg- 1;银杏叶提取物注射液A治疗各组分别在再灌注前 1h腹腔注射银杏叶提取物注射液A 5 ,10mg·kg- 1。测定脑组织中SOD ,GSH Px和MDA。结果 :2种注射液在不同的时间点均能明显提高脑组织中GSH Px和SOD(P <0 .0 5 )的活性 ,减少MDA(P <0 .0 5 ) ;2种注射液同等剂量差别无显著意义 (P >0 .0 5 )。结论 :银杏叶提取物注射液A可提高脑缺血再灌注大鼠的脑组织总抗氧化活力 ,降低脑组织中MDA含量 。AIM: To observe the effects of Ginkgo biloba extract (GbE)solution on SOD, GSH-Px and MDA in cortex of rats during cerebral ischemia and reperfusion. METHODS: Seventy-eight Sprague-Dawley (SD) rats were divided randomly into thirteen groups: sham control group, intraperitoneal injection of saline; ischemia-reperfusion groups, including ischemia (I) with 2 h/reperfusion(R) 1, 2, 3 h groups, injected intraperitoneally(ip) saline before reperfusion 1 h, respectively; Gb E A treatment groups, including I 2 h/R 1, 2, 3 h groups, ip Gb E A 5 mg·kg -1 before reperfusion 1 h, respectively; GbE B 5,10 mg·kg -1 treatment groups, including I 2 h/R 1,2,3 h groups, ip GbE B 5,10 mg·kg -1 before reperfusion 1 h, respectively. The model of focal cerebral ischemia-reperfusion was established with ligation of rat middle cerebral artery. Xanthine oxidase, DTNB and TBA were utilized for detecting activities of SOD, GSH-Px and contents of MDA in brain tissue, respectively. RESULTS: Comparing determining the ischemia-reperfusion control to treatment groups, GbE B injection can dose-dependently increase the activities of SOD and GSH-Px(P<0.05) and decrease contents of MDA(P< 0.05). There was no significant difference between GbE B 5 mg·kg -1 groups and GbE A 5 mg·kg -1 groups(P>0.05). CONCLUSION: GbE B injection could protect brain from ischemia-reperfusion injury with increase vitality to resist oxidation of brain tissue and decrease content of MDA.

关 键 词：再灌注损伤 动物 实验 丙二醛 超氧化物歧化酶 谷胱甘肽过氧化物酶 二裂银杏 杏花雨注射液 

分 类 号：R965.1[医药卫生—药理学]