VEGFA、VEGFC及其受体和angiopoietin-1/angiopoietin-2及其受体在发育第3-12周人胚胎肝的表达  

Expression of VEGFA, VEGFC, Angiopoietin-1, Angiopoietin-2 and Their Receptors on Development Liver During Gestation of Weeks 3-12 of Human Em bryo

作　　者：梅妍[1] 李爱冬[1] 蒋吉英[2] 周鸿鹰[2] 羊惠君[2] 杨淑霞[1] 洪华容 宋红瑞 

机构地区：[1]第三军医大学成都军医学院科研实验中心 [2]四川大学华西医学中心解剖教研室,成都610044

出　　处：《中国实验血液学杂志》2004年第5期632-636,共5页Journal of Experimental Hematology

摘　　要：本研究观察VEGFA和VEGFC及其受体和angiopoietin 1 angiopoietin 2及其受体在发育第 3- 12周人胚胎肝的表达。在征得意外流产孕妇同意并签字后 ,收集其意外流产受精龄为第 3- 12周的人胚胎 ,4 %多聚甲醛固定 ,石蜡包埋切片 ,HE和免疫组织化学染色 ,光镜观察。结果表明 :在发育第 4周 ,肝由少量肝索组成 ,肝内未见造血细胞和血细胞。肝内大、圆、有核的原始血细胞于发育第 5周开始出现 ,并强表达VEGFA、flt 4和Tie 2 ,其数量在发育第 7周达高峰。第 11- 12周 ,阳性细胞数量减少。这类细胞仅于发育第 6周时短暂表达Flk 1。第 7- 12周 ,肝细胞表达VEGFC和flt 1,阳性反应产物积聚在细胞质中。发育第 5周到第 12周胚胎肝血管内 ,有angiopoietin 1和angiopoietin 2阳性反应的细胞存在 ,细胞形态同上述VEGFA、flt 4和Tie 2阳性细胞。angiopoietin 1和angiopoietin 2阳性反应较弱 ,Tie 2的免疫反应强。发育各期 ,肝细胞也弱表达angiopoietin 1和angiopoietin 2和Tie 2。发育各期血管内皮细胞为上述各种因子和受体阴性反应。结论 :发育第 7周之前和第 7周之后 ,VEGF家族及其受体在肝内造血灶的细胞表达模式不同。胎肝造血与肝细胞的发育可能相关。The aim was to study the expression of VEGF-A, V EGF-C, angiopoietin-1, angiopoietin-2 and their receptors on development liv er during gestation of weeks 3-12 of human embryo. Human embryo contingently a borted at 3-12 weeks of gestation were collected with signed agreements of the p regnant women suffered from accidental abortions. The specimens were fixed by 4% par aformaldehyde and embedded by paraffin. 5 μm serial sections were made. HE sta ining, immunohistochemistry method and light-microscope were employed. The results sh owed that at 4-5 weeks of development, liver was constituted by a few hepatic co rd s. Hematopoietic cell or blood cells were undetectable in the 4 week of gestatio n. A few cells which were larger, rounded and nucleared cells appeared and expre ssed VEGFA, flt-4 and Tie-2 proteins strongly in liver at 5 weeks of gestation . The number of these immuno-positive cells was highest in the 7th week and dec re ased at 11-12 weeks of gestation. These cells expressed flk-1 transiently in th e 6th week. VEGF-C and flt-1 were expressed by hepatic cells from weeks 7 to 1 2 of gestation. The immuno-positive products were deposited in plasma of hepatic cells. Angiopoietin-1, angiopoietin-2 and Tie-2 were detectable on those cells whic h expressed VEGFA, flt-4 and Tie-2 from weeks 5 to 12 of gestation. The expres sion of angiopoietin-1 and angiopoietin-2 were weakly and Tie-2 was strongly. They were expressed weakly too by hepatic cells at 5 to 12 weeks of gestation. All factors and their receptors were undetectable on vascular endothelial cells at 4-12 weeks of gestation. It is concluded that the expression patterns of VEG F family on cells of liver are different before and after 7 weeks of gestation. The hematopoiesis in fetal liver may be related to development of hepatic cell.

关 键 词：造血 肝 胚胎 VEGF家族 ANGIOPOIETIN 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R331.2[医药卫生—基础医学]