二甲基亚砜联合羟乙基淀粉的非程控降温法冻存脐血造血干细胞研究  

Study on Non-programmed Process Using Dimethyl Sulfoxide and Hydroxyethyl Starch as Cryoprotectants in Cryopreservation of Cord Blood Hematopoietic Cells

作　　者：刘开彦[1] 董文川[1] 王逸兰[1] 姜永军[1] 高志勇[1] 黄宁伟[1] 陆道培[1] 

机构地区：[1]北京大学人民医院,血液病研究所,北京市脐血造血干细胞库,北京100044

出　　处：《中国实验血液学杂志》2004年第5期670-673,共4页Journal of Experimental Hematology

基　　金：北京市科委基金 ( 95 5 0 2 13 10 0 );国家重点基础研究( 2 0 0 1CB10 10 5 );2 11课题;985课题基金资助

摘　　要：为了探索有效低温保存脐血造血干 祖细胞的冷冻保护剂和技术 ,进行了二种方法试验。第一种方法联合使用二甲基亚砜 (DMSO)和羟乙基淀粉 (HES) (分子量 12 0 0 0 0 )作为冷冻保护剂并用非程控降温冷冻技术 ;第二种方法只使用DMSO作为冷冻保护剂并用程控降温仪冷冻技术保存脐血。对二种方法的效果进行比较。结果表明 :15份脐血冻存后细胞存活率、CFU GM回收率 ,无论在使用非程控降温的第一种方法和使用程控降温仪的第二种方法均无显著性差异。 183份脐血质量检测结果及 2 1例临床移植结果也显示两种方法无显著性差异。结论 :联合使用DMSO和HES作为冷冻保护剂并用非程控降温冷冻的方法对脐血造血干细胞有良好的保护作用 ,是一种简单易行、省时省力、适合于脐血造血干细胞库大批量冻存脐血的方法。This study was aimed to search for effective cryoprotectants and freezing metho ds used in cord blood bank (CBB) for cryopreservation of cord blood hematopoietic stem cells. The non-programmed group using 8% final concentration of dimethyl sulfoxide (DM SO)and 5% final concentration hydroxyethyl starch(HES)(molecular weight 120 000)as protectants and group of conventional of programmed controller method using 10% DMSO only as cryoprotectant in cryopreservation of co rd blood hematopoietic stem cells were compared. In each of the two groups, 15 c ord blood uni ts were used. In non-programmed group, cord blood units put in -80℃ refrigerat or for 24 hours as a transitional step before deep-freezing in liquid nitrogen, wh en both of DMSO and HES had been added. The recoveries of the nuclear cells numb er, the yield of granulocyto-macrophage colony forming units (CFU-GM) and the ce lls viability in cord blood units before preservation and after thawing were tes ted for both methods. The results showed that no significant difference was foun d in above assays betwee n two groups. The clinical application results also showed that hematopoietic en graftment rates after infusion were similar in both groups. It is concluded that the non-programmed method by -80℃ refrigerator as a transitional step and us in g the combined two protectants seems simple in operation and effective in clinic al transplantation as well as the conventional programmed method.

关 键 词：脐血 低温冻存 二甲基亚砜 羟乙基淀粉 造血干细胞 

分 类 号：R318.52[医药卫生—生物医学工程] R331.1[医药卫生—基础医学]