免疫介导再生障碍性贫血小鼠IL-3和IL-3受体变化的研究  

Study on the Changes of IL-3 and Its Receptor in Mice with Immune-mediated Aplastic Anemia

作　　者：黄韬[1] 黄振翘[1] 周永明[1] 薛志忠[1] 甘人宝[2] 

机构地区：[1]上海中医药大学附属岳阳中西医结合医院血液科,上海200437 [2]中国科学院上海生物化学研究所,上海200031

出　　处：《中国实验血液学杂志》2004年第5期684-686,共3页Journal of Experimental Hematology

摘　　要：本研究目的是为再生障碍性的贫血的临床治疗提供新的思路。建立了免疫介导再生障碍性贫血小鼠模型 ,用酶联免疫吸附法 (ELISA)测定植物血凝素 (PHA)刺激的再生障碍性贫血小鼠脾细胞培养上清液中IL 3,以点杂交法对其靶细胞受体进行半定量分析。结果表明 ,用植物血凝素 (PHA)刺激的再生障碍性小鼠脾细胞培养上清液中IL 3含量高于正常对照 ,二者之间有明显差异 (P <0 .0 0 1) ;其靶细胞IL 3受体的表达量明显低于正常对照。结论 :IL 3靶细胞的IL 3受体水平在再生障碍性贫血的治疗中起着重要的作用。The aim of this study was to find new idea for cl inical treatment of aplastic anemia. Immune-mediated aplastic anemia mice were developed, IL-3 in the supernatant with PHA stimulating splenic cells was det ected by ELISA, semi-quantiting analysis of IL-3R was performed by point hybri dization.The results showed that the IL-3 level in the supernatant with PHA stimulating splenic cells of immune-mediated aplastic anemia mice was high er than controls, difference between them was significant (P<0.001), while a mount of IL-3 receptor by semi-quantiting analysis was lower than control sig nificantly. In conclusion, the IL-3 receptor expression level is important for pathogenesis and treatment strategy of aplastic anemia.

关 键 词：免疫 再生障碍性贫血 动物模型 IL-3 IL-3R 

分 类 号：R556.5[医药卫生—血液循环系统疾病] R392.11[医药卫生—内科学]