免疫滴金技术检测肾综合征出血热特异性抗体与中西医结合治疗的研究  

A study on detecting specific IgM of hemorrhagic fever with renal syndrome with colloidal gold immuno-dot assay and of treating with traditional and western medicine

作　　者：储峰 季青 严润民 陈晓玲 王霞明 

机构地区：[1]上海市南汇县南华医院,201300

出　　处：《上海预防医学》1999年第12期544-547,共4页Shanghai Journal of Preventive Medicine

摘　　要：目的　探索一种更为简便、快速、特异、灵敏的肾综合征出血热( HFRS) 抗体的检测方法及中西医结合治疗该病的有效手段。方法　115 例HFRS 病人血清同时采用免疫滴金法(CGIDA) 与酶联免疫吸附法(ELISA)对比检测特异性免疫球蛋白M 抗体( 抗HFRS- IgM) 、免疫荧光法(IFAT) 对比检测特异性免疫球蛋白G 抗体( 抗HFRS- IgG) ,并以20 例发热待排、48 例病毒性肝炎血清对照;101 例HFRS 病人分组进行中西医结合治疗,治疗组用苦黄、参麦注射液联合黄芪汤,对照组用利巴韦林联合甘利欣注射液。结果　115 例HFRS 病人血清,以CGIDA法检测抗HFRS- Ig M,灵敏度77 .4 % ,特异度100 % ;以CGIDA 法检测抗HFRS- IgG,灵敏度90 .4 % ,特异度100 % 。治疗组50 例与对照组51 例用药后退热天数、主要症状、体征缓解天数相似( P> 0 .05) ,尿蛋白消失及肾功能恢复天数,对照组优于治疗组( P< 0 .05) ,在越期方面,越休克期治疗组优于对照组( P< 0 .05) ,越少尿期及从发热期直接进入多尿期,两组情况相似( P> 0 .05) 。结论　CGIDA 检测HFRS 特异性抗体具有快速、简便、灵敏、特异之优点。苦黄、参麦注射液联合黄芪汤治疗HFRS 与利巴韦林联合甘利欣注射液相比较,疗效无明显差别,但前者优于改善休克情况,后者强于改善肾功能。Objectives To explore a simple,speedy,specific and sensitive method to detect specific IgM (SIgM)and IgG(SIgG)antibody of hemorrhagic fever with renal syndrome(HFRS).To study the therapeutic effects of traditional and western medicine on HFRS. Method The serum of 115 patients with HFRS were tested with colloidal gold immuno-dot assay(CGIDA) for SIgM and SIgG antibody and compared with enzyme linked immunosorbent assay(ELISA)or indirect fluorescent antibody test (IFAT).101 patients with HFRS were randomized into a treatment group (n=50,Kuhuang and shenmai injection with Huangqi liquid)and a control group (n=51,Ribarvirin with Ganlixin injection). Results With CGIDA,the rate of specificity of detected SIgM was 100% and sensitivty was 77.4% detection of SIgG was 100% too,sensitivity was 90.4%. They were similar during the fever declined days,the symptoms alleviated days and sign relieved days between treatment group and control group.Days for recovery from albuminued and renewed kidney function,the treatment group was better than the control(P<0.05).The crossed shock state rate of the treatment group was higher than that of the control(P<0.05). Conclusion To detect the SIgM or SIgG antibody in serum of patients with HFRS,CGIDA was more simple and speedy,more specific and sensitive than ELISA or IFAT.About treating of HFRS,the effects of treatment group was similar to that of control group.But the crossed shock state rate in the treatment group was higher than that of control group,the renewed kidney function in the control group was better than that in the treatment group.

关 键 词：HFRS 对照组 治疗组 特异性抗体 肾综合征出血热 

分 类 号：R512.8[医药卫生—内科学] R554.6[医药卫生—临床医学]