肝素共混丝素蛋白膜上AT-的固定化及其体外抗凝血性能  

Immobilization of Antithrombin-Ⅲ on Fibroin/Heparin Blend Membrane and Its Anticoagulant Performance

作　　者：杨新林[1] 吴克友[1] 于群[2] 范翠红[1] 李茂林[1] 朱鹤荪[3] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081 [2]军事医学科学院野战输血研究所,北京100850 [3]北京理工大学材料科学与工程学院,北京100081

出　　处：《北京理工大学学报》2004年第9期837-840,共4页Transactions of Beijing Institute of Technology

基　　金：国家"九七三"计划项目(G1999064705)

摘　　要：用抗凝血酶(antithrombin- ,AT- )和肝素对生物材料进行表面改性,以提高其抗凝血性能.以低浓度肝素丝素共混蛋白膜为基质,利用等离子体处理辅助的共价交联方法对AT- 进行固定化.用过剩法对固定化效果进行评价,固定化后的活性采用体外凝血时间进行检测,人血管内皮细胞在材料上的生长情况用MTT法测定.结果显示,通过该方法可以有效地将蛋白固定化,低浓度肝素丝素共混膜固定化AT- 后,不仅其抗凝血性能有了一定的改善,而且与普通的肝素共混膜相比,细胞毒性也有所降低.该研究结果拓展了AT- 蛋白的应用范围,同时为抗凝血材料的设计提供了一种新的思路.A biomaterial silk fibroin is surface modified using antithrombin-Ⅲ(AT-Ⅲ) to improve its anticoagulant property. AT-Ⅲ was immobilized on the surface of silk fibroin/heparin blended membrane by NH_3 plasma treatment, followed by covalent cross-linking reaction. The immobilization efficiency was evaluated by protein surplus analysis method. The in vitro antithrombogenicity representing the activity of AT-Ⅲ was determined by the method of activated partial thromboplastin time(APTT), prothrombin time (PT) and thrombin time (TT) test. The cytotoxicity of materials was tested by the MTT method. The results demonstrated that the AT-Ⅲ is immobilized on the membrane in an efficient and activity preservant form and, the anticoagulant performance of the modified fibroin membrane improved. These results anticipater the wider application of AT-Ⅲ, and provide new visions on the design of anticoagulant materials.

关 键 词：丝素蛋白膜 AT-Ⅲ 肝素 固定化 细咆毒性 抗凝血材料 

分 类 号：O69[理学—化学] TB324[一般工业技术—材料科学与工程]