24例病毒性、15例化脓性脑炎患者脑脊液中乳酸脱氢酶及同功酶分析  

Analysis of LDH and its iso-enzyme in cerebralspinal Fluid of 24 virogenetic and 15 bacteritic cerebritis patients

作　　者：韩猛[1] 

机构地区：[1]天津市第一中心医院检验科,天津300192

出　　处：《天津医科大学学报》2004年第3期425-426,共2页Journal of Tianjin Medical University

摘　　要：目的 :观察病毒性、化脓性脑炎脑脊液(csf)中乳酸脱氢酶 (LDH)活性及其各同功酶活性百分率的变化。方法:病毒性脑炎24例 ,化脓性脑炎15例 ,酶动力学法检测脑脊液总乳酸脱氢酶 (TLDH)活性 ,琼脂糖凝胶电泳法检测脑脊液中LDH各同功酶活性百分率。结果:病毒性脑炎与对照组比较 ,脑脊液总乳酸脱氢酶 (TLDH)明显降低 (P<0.01) ,LDH1同功酶显著降低 (P<0.01) ,LDH5显著升高 (P<0.01) ;化脓性脑炎与对照组比较脑脊液TLDH明显升高 (P<0.01) ,LDH1同功酶明显降低 (P<0.01) ,LDH5明显升高 (P<0.01)。结论:脑脊液TLDH值的测定可以鉴别病毒性、化脓性脑膜炎 ,检测脑脊液LDH各同功酶活性 (LDH1和LDH5)的变化程度对脑炎的进展及预后有一定的价值。Objective:To determine the changes of the LDH and LDH isoenzyme activity of Cerebralspinal Fluid(csf)in virogenetic and bacteritic cerebritis.Methods:The enzyme kinetic method was used in LDH acˉtivity determination.The activity of LDH isoenzyme was determined with agarose electrophoresis.Results:TLDH of csf in virogenetic cerebritis was significantly lower than that of control(P<0.01).TLDH of csf in bacteritic cerebritis was significantly higher than that of control(P<0.01).The changes of LDH isoenzyme activity of csf in both virogenetic and bacteritic cerebritis were the same.Conclusion:TLDH of csf can be used to distinguish two kinds of cerebritis.LDH isoenzyme activity of csf might be valuable data in monitoring the progress and prognosis of cerebritis.

关 键 词：脑炎 脑脊液 乳酸脱氢酶 乳酸脱氢酶同功酶 

分 类 号：R446.14[医药卫生—诊断学]