无机碳与雨生红球藻(Haematococcus pluvialis)细胞调节物质  

INORGANIC CARBON AND THE CELL GROWTH REGULATOR INMICRO-ALGA HAEMATOCOCCUS PLUVIALIS

作　　者：刘建国[1] 孙艳妮[1] 殷明焱[1] 刘伟[1] 张展[1] 

机构地区：[1]中国科学院海洋研究所海洋生物技术研究发展中心,青岛266071

出　　处：《海洋与湖沼》2004年第5期459-466,共8页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;395 0 0 114号 ;39970 5 75号 ;国际科学基金项目 (IFS)资助 ;A/ 2 786 2号 ;国家农业成果转化基金资助项目 ;0 2EFN2 16 6 0 12 13号 ;中国科学院知识创新重要方向项目 ;L4 80 32 4 0 9D号 ;中国科学院海洋研究所创新项目 ;L86 0 32 5

摘　　要：以单细胞雨生红球藻为材料 ,采用酸碱滴定和CO2 加富通气培养微藻的方法 ,对旧液中HCO-3 和CO2 -3 浓度变化以及对红球藻细胞生长的影响进行了研究。结果表明 ,旧液具有限制红球藻细胞生长和诱导细胞转化的作用。同时 ,旧液中无机碳离子浓度明显高于新液。培养液中富含CO2 -3 时 ,各细胞数量与CO2 -3 浓度呈正相关 ,相关系数为 0 .88。溶液中仅有HCO-3 时 ,各细胞数与HCO-3 浓度也呈正相关性。因此 ,排除了CO2 -3 和HCO-3 作为旧液中的调节物质 ,限制红球藻细胞生长和诱导细胞转化的可能性。旧液乙酸乙酯提取物生物检测实验表明 ,在粗提取物中有降低细胞增长和诱导细胞转化的活性 ,表明调节物质能溶于有机相 ,也反过来证实无机碳离子不是旧液中的调节物质。DNA含量和倍性分析结果表明 ,红球藻游动细胞DNA复制可以加倍后不经过原生质分裂就可以再次进行 ,因此推测旧液中的调节物质对原生质分裂过程产生抑制作用 ,而不对DNA复制过程产生抑制作用。Auto-regulation, probably a common phenomenon in micro-algae cultivation has been long overlooked in the low-cell-density cultures, especially at early culture stages because of the low auto-regulator concentration. In high-cell-density culture, however, auto-regulation become an important issue and occurs heavily at the later culture stages. To date, scientists are unsure exactly what kind of substance the auto-regulator is. Our early data has revealed an unknown bioactive substance produced by Haematococcus cell that feedback influences cell growth and transformation and effectively regulates cell cycles. About 60 years ago, Pratt (1942, 1944) and Pratt et al (1940, 1945) showed some auto-produced signal substances termed chlorellin feedback regulating Chlorella cell growth. Mandalam et al (1995) considered autoinhibitor does not exist in the old culture, and it is inorganic carbons play the inhibitory role on Chlorella growth. In this study, the relationship of HCO - 3 or CO 2- 3 to cell growth and cell transformation of H. pluvialis in static, aerated and/or CO 2-enriched aerated culture was investigated comparatively in Qingdao from 1999 to 2002 using a fresh medium and an old culture supernatant with equal content of nutrients. Results of CO 2-enriched aerated culture showed that motile cell growth rate in Haematococcus old culture was reduced, meanwhile cell transformation from motile cells to non-motile cells accelerated. These results imply that some unknown substances, such as an auto-regulator, existed in the old culture blocking Haematococcus motile cell division and inducing the process of cell transformation. Although CO 2- 3 and HCO - 3 concentration in supernatant of Haematococcus old culture was similar to that in Chlorella old culture supernatant, and the growth rate of motile cells in the old culture was significantly affected (much lower than that of fresh medium), further studies did not support a positive connection between inorga- nic carbons (CO 2- 3 or

关 键 词：雨生红球藻 粗提取物 调节物质 CO3^2- HCO3^- 

分 类 号：Q2[生物学—细胞生物学]