吸水链霉菌NND-52-C基因工程宿主载体系统的构建  

Establishment of Host and Vector System for Genetic Engineering of S.hygroscopicus NND-52-C

作　　者：江曙[1] 陈代杰[2] 戈梅[2] 黄为一[1] 

机构地区：[1]南京农业大学微生物学系,南京210095 [2]上海来益生物药物研究开发中心,上海201203

出　　处：《微生物学通报》2004年第5期38-42,共5页Microbiology China

基　　金：国家教委高教司博士学科点专项科研基金资助 (No 93 0 2 1 )

摘　　要：吸水链霉菌NND 5 2 C菌株是大环内酯类抗生素 阿扎霉素B的高产菌株。采用原生质体转化技术 ,将来自变铅青链霉菌TK2 4菌株的pIJ70 2质粒转化吸水链霉菌NND 5 2 C菌株的原生质体 ,建立了吸水链霉菌NND 5 2 C菌株的基因工程宿主载体系统。确定了NND 5 2 C菌株原生质体制备和再生的条件 ,其原生质体形成率达到 1 0 8个 mL ,再生率约为 0 2 % ,转化率为 1 0 2 ～ 1 0 3个转化子 μg质粒DNA。S. hygroscopicus NND-52-C is a high production strain of azalomycin B.A transformator was obtained when pIJ702 from S.lividans TK24 was used to transform the protoplast of S. hygroscopicus NND-52-C by the protoplast transformation technology.So host and vector system for genetic engineering of NND-52-C strain was established.And the protoplast formation and regeneration conditions were determined.As a result,protoplast formation rate and regeneration rate reached 108/mL and 0.2%,respectively.Transformation rate was 102～103/μg plasmid DNA.

关 键 词：吸水链霉菌NND-52-C菌株 阿扎霉素B 原生质体转化 宿主载体系统 

分 类 号：Q93[生物学—微生物学]