体外三维抗血管生成药物筛选模型的建立  

Construction of a three-dimensional human angiogenesis model in vitro for antiangiogenic drug selection

作　　者：李斌[1] 

机构地区：[1]云南大学药学院,云南昆明650091

出　　处：《第一军医大学学报》2004年第10期1167-1170,共4页Journal of First Military Medical University

基　　金：云南大学理(工)科校级科研项目资助(K1059069)

摘　　要：目的建立一种新的用于筛选具有抗血管生成作用的体外血管生成模型。方法人脐静脉的内皮细胞(HUVECs)在三维胶原纤维系统中培养,制备成具有毛细血管样网状结构模型;3种酪氨酸酶抑制剂genistein, tyrphostin A23和lavendustin C被分别加入到模型中,观察它们对血管形成的影响。结果在不加入任何生长因子的条件下, HUVECs 能迅速重组形成毛细血管样网状结构;3种酪氨酸酶抑制剂均对血管形成有抑制作用。结论该模型具有操作简单、可重复性好、易量化的特点,是一种在体外筛选血管生成抑制剂的理想模型。Objective To develop a simple human angiogenesis model that simulates all the critical steps of the angiogenic process for screening antiangiogenic substances. Methods Human umbilical vein endothelial cells (HUVECs) were sandwiched into three-dimensional collagen gels for culturing to produce the angiogenesis model in vitro, on which the antiangiogenic effects of genistein, tyrphostin A23 and lavendustin C were assessed. Results HUVECs had the capacity to repidly form highly organized capillary-like structures in this model with uniformity of the entire culture, and was easily reproducible. Tyrosine kinase inhibitors, genistein, tyrphostin A23 and lavendustin C were found to inhibit the capillary tube formation. Conclusion This new human angiogenesis model has proved to be simple, responsive, and reproducible for testing potential angiogenic inhibitors, and is suitable for screening anti-angiogenic drugs.

关 键 词：内皮细胞 血管生成 模型 药物筛选 

分 类 号：R965.1[医药卫生—药理学]