过氧乙酸消毒剂中多组分的快速反相液相色谱测定  

Rapid Reversed-Phase Liquid Chromatographic Determination of Components in Peroxyacetic Acid Disinfectant

作　　者：郭治安[1] 赵景婵[1] 张小辉[1] 曲波[1] 

机构地区：[1]西北大学化学系,西安710069

出　　处：《分析化学》2004年第10期1329-1332,共4页Chinese Journal of Analytical Chemistry

基　　金：陕西省教委基金资助项目 (No .0 3JK0 75 )

摘　　要：用反相C18色谱柱 ,采用离子抑制色谱法 ,在不经过衍生的情况下 ,同时测定了过氧乙酸消毒剂中过氧乙酸 (PAA)、H2 O2 (H2 O2 )、乙酸 (HAc)的含量。本方法以酸性磷酸盐为缓冲溶液 ,抑制PAA和HAc在流动相中的离解 ,提高其容量因子 ,使组分间能达到很好的分离 ;利用磷酸盐缓冲溶液在紫外 2 10nm以下吸收强度较水低的特性 ,降低了基线噪音 ,提高了过氧化物和羧酸测定的灵敏度。各组分的检出限分别为 2 .3ng (PAA) ,2 .1ng (H2 O2 )和 30ng (HAc)。Ion-suppression chromatography was applied in reversed phase chromatography. The peroxyacetic acid (PAA) hydrogen peroxide (H2O2) and acetic acid (HAc) were determined in the same injection without any assistance of derivation. The method based on the dissociation of sample was suppressed in acid medium. The phosphate buffer solution (PBS) was used to control dissociation of PAA and HAc. The capacity factors were improved and the samples were separated respectively. Because of PBS having absorption in ultraviolet 200 similar to 210 nm weakly as so as water was, thin the baseline noise was down. The determining sensitivity of peroxides and carboxylic acid was improved. PAA's minimum detectable quantity was 2.3 ng, thus corresponding to PAA concentration of 23 mug/L, H2O2's was 2.1 ng corresponding to H2O2 concentration of 21 mug/L, HAc's was 30 ng corresponding to HAc concentration of 300 mug/L. The sample was analysed in five minutes.

关 键 词：过氧乙酸消毒剂 快速测定 反相液相色谱 过氧乙酸 乙酸 过氧化氢 

分 类 号：O657.7[理学—分析化学]