间接荧光抗体试验诊断肠螨病初探  

Preliminary study on indirect fluorescent antibody test in diagnosis of intestinal acariasis

作　　者：冯忠伟[1] 崔玉宝[2] 邢应如[1] 

机构地区：[1]安徽省淮南第三矿工医院检验科,232001 [2]安徽理工大学医学院

出　　处：《临床输血与检验》2004年第4期252-253,共2页Journal of Clinical Transfusion and Laboratory Medicine

摘　　要：目的　探讨间接荧光抗体试验在肠螨病诊断中的应用价值。方法　采集 48例肠螨病患者血清 ,以粉尘螨 (成螨 )体组织切片为抗原 ,建立间接荧光抗体试验检测血清螨特异性抗体 Ig G。结果　肠螨病患者血清抗体反应滴度为 1∶ 4～ 1∶ 5 1 2 ,若以抗体滴度≥ 1∶ 1 6为阳性 ,阳性检出率达 91 .67% ( 4 4/48)。结论　间接荧光抗体试验诊断肠螨病具有一定的灵敏性 ,以血清抗体滴度≥ 1∶ 1Objective To evaluate the practical value of indirect fluorescent antibody test (IFAT) in diagnosis of intestinal acariasis. Methods Adult mite of dermatophagoides farinae were used as antigen, and IFAT was applied to detect mite-specific IgG in sera of 48 patients with intestinal acariasis. Results The antibody titers of positive reaction were from 1∶4 to 1∶512 in patients with intestimal acariasis. If antibody titer over 1∶16 was regarded as positive, the positive rate of the patients detected by IFAT was 91.67% (44/48).Conclusion IFAT is a sensitive method for diagnosis of intestinal acariasis and antibody titers of 1∶16 or more should be regarded as positive.

关 键 词：间接荧光抗体试验 诊断 肠螨病 

分 类 号：R446.62[医药卫生—诊断学] R53[医药卫生—临床医学]