毛细管气相色谱法测定海带提取多糖中的L-褐藻糖含量  

Determination of L-fucose content in polysaccharide extracted from Laminaria japonica using capillary gas chromatography

作　　者：赵增芹[1] 刘希光[1] 牛锡珍[1] 张虹[1] 张全斌[1] 李智恩[1] 

机构地区：[1]中国科学院海洋研究所

出　　处：《海洋科学》2004年第10期10-13,共4页Marine Sciences

基　　金：中国科学院方向性创新项目(KZCX3-SW -215 ;02-2-kj-hh-71(2003)10)

摘　　要：建立快速而准确灵敏的方法测定海带 (Laminariajaponica)提取多糖———褐藻多糖硫酸酯中的L 褐藻糖含量。海带多糖经酸解后 ,转化成糖腈乙酸酯衍生物。采用SGEAC225毛细管柱进行分离 ,实验中以肌醇为内标物。从青岛产海带提取的褐藻多糖硫酸酯中仅含有L 褐藻糖和D 半乳糖2种单糖 ,样品10 1,10 2,10 3 ,10 4中的L 褐藻糖的平均含量分别为32.9072 % ,32.3147% ,31.2924% ,32.3571% ,回收率为97.1%～99.9% ,变异系数为0.23 %～0.60%。采用气相色谱法测定海带多糖中的L 褐藻糖含量准确、可靠。To establish a better method for determining the content of L-fucose in fucoidan samples,a polysaccharide was extractedfrom Laminaria japonica.Hydrolysis ofthe polysaccharides was carried outwith2mol/LCF 3 COOH.Hydrolyzates were derivatized tothe aldononitrile acetates and analyzed byGCusinga capillarycolumn on a HP5890Ⅱgas chromatography equipedwitha flame ionizationdetector(FID).Myo-inositol was used as internal standard.Nitrogenwas used as the carriergas with a flowrate of1mL/min and a split ratio of100∶1.Chromatography runs at isothermal215℃,while the injector and detector were set at250℃.The polysaccharide was composed of L-fucose and D-galactose.The contents of L-fucose in the selected fucoidan samples were about32%with the recovery from97.1%to99.9%and the coefficient of variaton from0.23%to0.60%.The results showed thatthe gas chromatographic method fordeterminingthe contentofL-fucose in fucoidan is convenient and reliable.

关 键 词：海带(Laminaria japomica) 褐藻多糖硫酸酯 L-褐藻糖 气相色谱法 

分 类 号：O658[理学—分析化学]