藏药柳茶药材的HPLC指纹图谱研究  

The HPLC Fingerprint Analysis of Sibiraea angustata

作　　者：杨梅[1] 王天志[1] 濑井康雄 王晓静[1] 陶婷婷[1] 

机构地区：[1]四川大学华西药学院生药学教研室,成都610041 [2]イスクラ产业株式会社技术中心

出　　处：《中国天然药物》2004年第5期301-303,共3页

摘　　要：目的 :采用反相高效液相色谱法 ,研究并建立藏药柳茶药材的指纹图谱。方法 :采用岛津公司CLC ODS柱 (6 0mm× 15 0mm ,5 μm) ,甲醇 0 1%磷酸 (33∶6 7)洗脱 ;流速 1 0ml/min ;检测波长 2 2 0nm。 结果 :所得的色谱图各色谱峰分离较好 ,基线平整 ,达到指纹图谱的要求。结论AIM: To study and establish the fingerprint of Sibiraea angustata by RP HPLC.METHOD: The chromatographic conditions were as follows:a CLC ODS column(6 0 mm×150 mm,5 μm)was used;the mobile phase was methanol -0 1% phosphate(33∶67);the flow rate was 1 0 ml/min and the UV absorbance detection was set at 220 nm RESULT:Under the selected chromatographic conditions,good HPLC fingerprints of Sibiraea angustata were obtained CONCLUSION: Quality of Sibiraea angustata can be controlled effectively by HPLC fingerprint

关 键 词：藏药 HPLC指纹图谱 药材 色谱图 反相高效液相色谱法 检测波长 磷酸 甲醇 分离 

分 类 号：R284[医药卫生—中药学] R282[医药卫生—中医学]