体外培育牛黄与天然牛黄指纹图谱的比较研究  

Comparative Study on Fingerprints of Cultured and Natural Calculus Bovis

作　　者：丁岗[1] 盛龙生[2] 李明慧[1] 张弦[1] 蔡宝昌[1] 

机构地区：[1]江苏中康药物科技有限公司,南京211100 [2]中国药科大学分析测试中心,南京210009

出　　处：《中国天然药物》2004年第5期309-312,共4页

摘　　要：目的 :研究、比较体外培育牛黄与天然牛黄的指纹图谱。方法 :利用TOFMS、HPLC/MS技术研究、比较体外培育牛黄与天然牛黄中肽类、胆汁酸类和胆红素类 3类成分的指纹图谱。结果 :体外培育牛黄与天然牛黄 3类成分的指纹基本一致 ,但各成分间的相对含量有差异。结论 :10批体外培育牛黄的指纹图谱有较好的一致性 ,说明体外培育牛黄的质量稳定。 4批天然牛黄的指纹图谱 ,尤其是胆汁酸类成分的指纹图谱差异明显 ,说明天然牛黄由于来源不同 ,导致质量不够稳定。AIM: To study the fingerprints of cultured and natural Calculus Bovis METHOD:Analysis and comparison were made for the fingerprints of peptides,bile acids and bilirubins of cultured and natural Calculus Bovis by using TOFMS and HPLC/MS techniques RESULT:The fingerprints of peptides,bile acids and bilirubins of cultured Calculus Bovis were similar to those of natural Calculus Bovis,but differences exist in the relative content of each constituent CONCLUTION:The fingerprint repeatability of 10 batches cultured Calculus Bovis indicated that the quality of cultured Calculus Bovis was constant The fingerprints of 4 batches natural Calculus Bovis,especially the fingerprints of bile acids revealed that the quality of natural Calculus Bovis was not constant because of different origins

关 键 词：体外培育牛黄 指纹图谱 天然 胆汁酸 肽类 胆红素 成分 相对含量 

分 类 号：R282[医药卫生—中药学] R284[医药卫生—中医学]