甘露醇对动物血清胆碱酯酶的影响  

Effect of Mannitol on the Activity of Serum Cholinesterase in the Mouse and Rabbit

作　　者：刘翠霞[1] 吴基良[2] 江南[1] 贾贝[3] 毛洪涛[3] 江涛[3] 董伟杰[3] 

机构地区：[1]湖北中医学院药学系,武汉430064 [2]咸宁学院,437000 [3]湖北中医学院2001级本科班,武汉430064

出　　处：《医药导报》2004年第10期714-716,共3页Herald of Medicine

摘　　要：目的 :研究甘露醇对小鼠和家兔外周血清胆碱酯酶的影响。方法 :取小鼠 3 6只、家兔 3 2只均随机平分为 4组。A组给予 2 .5 %敌百虫 5 0 0mg·kg 1 ,小鼠用腹腔注射 ,家兔用耳缘静脉注射 ;B组给予 2 0 %甘露醇 2 0 0mg·kg 1 ,iv ,C组给予 10 %甘露醇 10 0mg·kg 1 ;D组给予等容量 0 .9%氯化钠注射液 ,iv。A组于给药 10min ,其他组静脉注射 3 0min后 ,采血测定血清胆碱酯酶活性。结果 :B、C组的小鼠、家兔胆碱酯酶活性明显低于D组 ,但高于A组。结论 :甘露醇可抑制血清胆碱酯酶活性 ,抑制作用具有剂量依赖性 ,但抑制作用低于敌百虫。Objective:To study the effect of mannitol on the activity of serum cholinesterase in the mouse and rabbit. Methods:36 mice and 32 rabbits were randomly divided into 4 groups:A,B,C and D, separately. Mice of group A were given each an intrapeitoneal injection of 500 mg·kg -1 of 2.5% dipterex, while those of group B,C and D were given each an intravenous injection of 200 mg·kg -1 of 20% mannitol, 100 mg·kg -1 of 10% mannitol and equal volume of 0.9% sodium chloride solution,respectively. Rabbits of group A,B,C and D were given each an intravenous injection of 500 mg·kg -1 of 2.5% dipterex, 200 mg·kg -1 of 20% mannitol, 100 mg·kg -1 of 10% mannitol and equal volume of 0.9% sodium chloride solution, respectively. 10 min after the injections of dipterex in mice and rabbits of group A,30 min after the intravenous injections in animals of group B,C and D,blood samples of all the rodents studied were collected for the determination of serum cholinesterase activity with the Ellman's method. Results:Serum cholinesterase activities of the mice as well as the rabbits of group B and C injected with mannitol were lower than those of the group D injected with normal saline(P<0.05), but higher than those of the group A injected with dipterex(P<0.05). Conclusion:Mannitol was shown to exert an inhibitory effect on the serum cholinesterase activity in mice and rabbits in a dose dependent manner.However,the effect was weaker than that of dipterex.

关 键 词：甘露醇 敌百虫 血清胆碱酯酶 抑制率 

分 类 号：R983.1[医药卫生—药品] R965[医药卫生—药学]