日本血吸虫亲免素基因的扩增及序列分析  

Schistosoma japonicum: Amplification and Sequence Analysis of Novel Gene Encoding Immunophilin

作　　者：李孜[1,2] 余新炳[1] 吴忠道[1] 徐劲[1] 田春林[3] 

机构地区：[1]中山大学基础医学院寄生虫学教研室 [2]广州医学院微生物与免疫学教研室,广东广州510182 [3]广西医科大学

出　　处：《中山大学学报（医学科学版）》2004年第5期395-398,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金资助项目(30070683);国家教育部博士点项目(200045);广东"211工程"重点建设基金资助项目(98169)

摘　　要：【目的】研究日本血吸虫亲免素基因编码序列的结构及进行初步的功能预测。【方法】用5'端和3'端锚定PCR从尾蚴文库中扩增,以获取日本血吸虫中国大陆株亲免素基因完整的开放阅读框(openreadingframe,ORF);用生物信息学技术确认其是否为亲免素编码基因并进行结构功能的初步分析和预测。【结果】用5'端和3'端锚定PCR从尾蚴文库中获得了亲免素基因5'端和3'端所缺序列,得到的亲免素基因cDNA的总序列长为1438bp,其ORF长1296bp,编码431个氨基酸。利用生物信息学技术鉴定其为日本血吸虫亲免素基因的完整cDNA序列。并用RT-PCR从日本血吸虫尾蚴mRNA扩增出亲免素基因的ORF。【结论】成功获得了日本血吸虫亲免素编码基因的全长cDNA,为进一步的功能研究打下了基础。To learn the coding sequence structu re of immunophilin gene of Shistosoma japonicum(Sj )and predict its funtion.The full-length cDNA of immunophili n gene was obtained by 5'-end and 3'-end nested polymerase chain reaction(PCR).Specific primers were synthesized and used to amplify immu nophilin gene by reverse transcript ion PCR(RT-PCR)from Sj cercariae mRNA.The product of PCR wa s sent to Bioasia company to sequence.The cDNA sequences were analyzed by bioinformatics.A novel cDNA sequence was obtained,t he sequence contains a full-length seq uence with 1296bp open read frame (ORF),which encodes 431amino acid residues.The new gene was identified to be the gene encoding im munophilin.Its sequences were identified by sequen cing and bioinformatics.[Conclusion]The full-length cDNA sequences encoding Sj immunophilin were firstly obtained,which gave the basis for further stu dy.

关 键 词：日本血吸虫尾蚴 编码基因 扩增 生物信息学技术 尾蚴 日本血吸虫中国大陆株 PCR 功能预测 文库 序列 

分 类 号：R383.24[医药卫生—医学寄生虫学] R532.21[医药卫生—基础医学]