良、恶性胸腔积液脱落细胞端粒酶hTERT基因表达的检测  

Detection of telomerase hTERT Gene Expression in Exfoliated Cell in Benign and Malignant Pleural Effusion

作　　者：张湘燕[1] 冯端兴[1] 邓小林[2] 刘维佳[1] 刁晓源[1] 罗溶溶[1] 李云[1] 张小润[1] 贾美琳[3] 吴瑞明[1] 

机构地区：[1]贵州省人民医院呼吸内科,贵阳550002 [2]贵州省人民医院检查中心,贵阳550002 [3]贵州省人民医院病理科,贵阳550002

出　　处：《贵州医药》2004年第10期867-869,共3页Guizhou Medical Journal

摘　　要：目的通过检测良、恶性胸腔积液脱落细胞端粒酶hTERT基因的表达水平 ,探讨其在良、恶性胸腔积液中的诊断价值。方法采用相对定量RT PCR法检测 2 6例恶性胸腔积液 ,16例结核性胸腔积液中端粒酶hTERT基因的表达水平。结果恶性胸腔积液中端粒酶hTERT基因的表达水平明显高于良性胸腔积液 (P <0 0 5 )。结论端粒酶hTERT基因参与了恶性胸腔积液的病理生理过程。采用RT PCR法检测胸腔积液中端粒酶hTERT基因表达水平有助于临床上良、恶性胸腔积液的鉴别诊断。Objective To investigate the diagnostic significance of the detection of the expression of telomerase hTERT gene in benign and malignant pleural effusion. Methods Telomerase hTERT gene expression of exfoliated cell in pleural effusion of 16 cases with tuberculose pleural effusion and 26 cases with malignant pleural effusion were measured by RT-PCR.Results Telomerase hTERT gene expression of exfoliated cell in malignant pleural effusion was higher than that in tuberculose pleural effusion(P<0.05).Conclusion Telomerase hTERT gene might involve in pathological process of malignant pleural effusion.The detection of telomerase hTERT gene by RT-PCR is useful to diagnosis of pulmonary cancer and distinguish the benign and malignant of pleural effusion.

关 键 词：恶性胸腔积液 HTERT基因 端粒酶 脱落细胞 表达水平 良性 结核性胸腔积液 RT-PCR法 

分 类 号：R561[医药卫生—呼吸系统] R730[医药卫生—内科学]