Ⅱ型胶原介导的细胞毒免疫学实验  

Detection of Type Ⅱ Collagen-mediated Cytotoxic Immunology

作　　者：沈雁[1] 陈涛[2] 唐毅[1] 钟灿灿[1] 梁佩红[1] 黄雪芳[1] 邹海燕[1] 陈鸿辉[1] 

机构地区：[1]暨南大学医学第四附属医院(广州市红十字会医院)创伤外科研究所,广东广州5102402 [2]广州医学院第一附属医院,广东广州510220

出　　处：《国际医药卫生导报》2004年第20期8-10,共3页International Medicine and Health Guidance News

摘　　要：目的检测用猪Ⅱ型胶原免疫新西兰大白兔的细胞免疫状态。方法用Ⅱ型胶原免疫新西兰大白兔60天,定期抽取血浆检测抗Ⅱ型胶原抗体;第60天取兔的外周血淋巴细胞,取兔脾细胞、淋巴结分离淋巴细胞,进行体外二次Ⅱ型胶原刺激,检测由此引起的反应性的细胞增殖规津。实验分为二组,第一组加入不同浓度PHA作阳性对照,并测定非特异性免疫;第二组加入不同浓度Ⅱ型胶原,检测特异性免疫。结果正常兔的淋巴细胞在PHA剌激下发生增殖,但对Ⅱ型胶原的第一次剌激不发生增殖,而免疫兔对PHA和Ⅱ型胶原的剌激均能发生显著的增殖。结论表明异种Ⅱ型胶原在一定浓度下,可以引起免疫兔的抗Ⅱ型胶原抗体的升高,并可引起兔脾、外周血淋巴细胞增殖,异种Ⅱ型胶原能在体内引起细胞免疫。Objective To detect the cellular immunity state of New Zealand white rabbit immunized by pig type Ⅱ collagen. Methods The New Zealand white rabbit was immunized by type Ⅱ collagen for sixty days. The plasma were collected at a regular interval and the anti-type Ⅱ collagen antibodies were examined. At the sixtieth day, the peripheral circular lymphocytes and the lymphocytes separated from rabbit’s spleen cells and lymph nodes were collected and were stimulated by type Ⅱ collagen in vitro again. The regulation of reactive cellular proliferation caused by the stimulation was detected. The experiment samples were divided into two groups. The first group was the positive control group by adding different concentrations of PHA and the non-specific immunity was assayed. The different concentrations of type Ⅱ collagen were added to the second group and the specific immunity was assayed. Results The lymphocytes of normal rabbits show proliferation by PHA stimulation but no proliferation by the first stimulation of type Ⅱ collagen is observed. Obvious proliferation due to the stimulation of both PHA and type Ⅱ collagen in the immunized rabbit can be observed. Conclusion It shows that certain concentration of heterogeneous collagen may lead to an increase of anti-type Ⅱ collagen antibody in immunized rabbit and may cause a proliferation of rabbit spleen and peripheral lymphocytes. The heterogeneous type Ⅱ collagen causes cellular immunity in vivo.

关 键 词：抗Ⅱ型胶原抗体 增殖 刺激 细胞毒 外周血淋巴细胞 异种 大白兔 新西兰 实验 

分 类 号：R392[医药卫生—免疫学]