急性呼吸综合征患者外周血树突状细胞表型和功能的变化  

The alteration of phenotype and function of peripheral dendritic cells in severe acute respiratory syndrome

作　　者：刘树人[1] 张雁[1] 陈汉生[1] 谢庆[1] 曾水娣[1] 王添章[1] 李灼亮[1] 孔祥平[1] 

机构地区：[1]中国人民解放军全军传染病中心传染病科,广州510600

出　　处：《中华急诊医学杂志》2004年第9期616-618,共3页Chinese Journal of Emergency Medicine

摘　　要：目的　探讨严重急性呼吸综合征病毒 (SARSV)感染对树突状细胞 (DC)表型和功能的影响。方法　采用流式细胞仪检测SARS发病期患者 (9例 )和正常人 (7例 )DC的表型 ;分离培养DC和T淋巴细胞 ,用混合淋巴细胞反应检测SARS患者和正常人DC对T淋巴细胞增殖的诱导作用。结果　SARSV感染抑制了DC类刺激分子CD80、CD86和HLAⅡ类分子HLA DR以及与DC成熟有关的分子CD83的表达 ;抑制DC诱导T淋巴细胞增殖的能力。结论　SARSV可抑制DC的分化成熟和抗原递能力。Objective To explore the pathogenesis of severe acute respiratony syndrome(SARS)Methods The changes of phenotypes of dendritic cells (DCs) were assessed with flow cytometry in 9 SARS patients and 7 healthy persons.The DCs and T-lymphocytes were isolated with gradient centrifugation and cultured in vitro. The proliferation inductions of DCs to T-lymphocytes were measured with mixed lymphocyte reaction.Results Severe acute respiratory syndrome virus suppressed the expression of costimulatory molecules (CD80, CD86), HLA class Ⅱ(HLA-DR) and maturation associated molecule (CD83) on DCs,with significant difference between the two groups,P<0.05. SARS virus inhibited the proliferation function of DC on T-lymphocytes (P<0.05).Conclusion SARS virus can suppress the differentiation and maturation,and the antigen presentation functions of DCs.

关 键 词：DC 表型 患者 T淋巴细胞增殖 外周血树突状细胞 正常人 急性呼吸综合征 成熟 发病期 分离培养 

分 类 号：R511.9[医药卫生—内科学]