大鼠脊髓再灌注损伤中三甲氧苄嗪的保护作用  

The Protective Effect of Trimetazidine on Reperfusion Injury of Spinal Cord in Rats

作　　者：朱晓峰[1] 史宏宇[1] 马宝红[1] 邹志田[1] 龙村[2] 

机构地区：[1]佳木斯大学附属第一医院胸心外科,黑龙江佳木斯154002 [2]阜外心血管病医院体外循环科,北京100037

出　　处：《中国体外循环杂志》2004年第3期163-165,172,共4页Chinese Journal of Extracorporeal Circulation

基　　金：国家自然基金 (30 170 933)

摘　　要：目的　评价三甲氧苄嗪对大鼠脊髓再灌注损伤的保护作用 ,并探讨其作用机制。方法　 4 5只SD大鼠随机分为 3组 ,假手术组、对照组和三甲氧苄嗪组 ,每组 15只。建立大鼠脊髓缺血损伤模型 ,假手术组不阻断主动脉 ,对照组在肾动脉以下及髂动脉以上同时阻断主动脉 2 0min ,三甲氧苄嗪 (TMZ)组于主动脉阻断前 10min静脉内注射三甲氧苄嗪 (3mg/kg) ,余同对照组 ,2 0min后开放主动脉。分别于阻断主动脉前、松开主动脉前和松开主动脉后2h取静脉血测定丙二醛 (MDA)含量。术后 4 8h按Tarlov评分标准评价动物后肢神经功能 ,然后处死动物 ,取脊髓进行含水量、MDA含量测定及组织病理学检查。结果　TMZ组血中MDA含量明显低于对照组 (P <0 .0 5 ) ;TMZ组动物后肢神经功能评分明显高于对照组 (P <0 .0 1) ;TMZ组脊髓含水量、MDA含量明显低于对照组 (P <0 .0 1) ;TMZ组光镜下脊髓病理改变轻微 ,而对照组较重 ,两组病理评分有显著性差异 (P <0 .0 1)。OBJECTIVE To evaluate the effect of trimetazidine on reperfusion injury of spinal cord in rats and discuss its mechanism .METHODS With rat spinal cord ischemia model , we induced ischemia by clamping the aorta just distal to the renal artery and above the iliac bifurcation for 20 min . Forty-five spraque-dawley rats were randomly assigned to three groups(n=15 in each group ) . Sham-operation group subjected to operation dissections without aortic occlusion ; control group undergoing occlusion but receiving no treatment ; TMZ (trimetazidine)group receiving 3mg/kg trimetazidine intravenously in ten minutes before the occlusion of aorta. The malondialdehyde(MDA) content in blood was measured before the occlusion, during the ischemia and after 2 hours of reperfusion respectively. Neurologic status was assessed in 48h after operation , then all animal were sacrificed and the water content , MDA content of spinal cord were also measured . The spinal cord was processed for histopathologic examination . RESULTS Compared with the control group , the TMZ group had lower water content and MDA content (P<0.01) . The neurological scoring of TMZ group was significantly higher than that of control group (P<0.01) . The histopathologic change of spinal cord in TMZ group was milder compared with that in control group (P<0.01) . CONCLUSION Trimetazidine could protect the rat spinal cord from ischemic-reperfusion injury .

关 键 词：对照组 三甲氧苄嗪 TMZ 主动脉 脊髓 大鼠 保护作用 再灌注损伤 阻断 手术 

分 类 号：R651.2[医药卫生—外科学]