鼠成骨细胞体外培养方法及初步观察  

METHODS AND OBSERVATIONS ON RAT CALVARIAL CELL CULTURE IN VITRO

作　　者：顾琪[1] 甘洁民[1] 仇志军[1] 吴衡[1] 韩瑞平[1] 周筱倩[1] 施泓[1] 杨振球 沈倩[1] 汪家愉[1] 

机构地区：[1]华东医院上海老年医学研究所,上海市200040

出　　处：《老年医学与保健》1999年第2期63-64,共2页Geriatrics & Health Care

基　　金：院级科研课题基金资助95H-72

摘　　要：目的 建立体外培养成骨细胞的方法.方法 应用机械和酶解方法,从新生大鼠颅骨分离培养成骨细胞.用偶氮染色法和钙钻法检测细胞碱性磷酸酶活性;Von Kossa染色显示细胞结节内有钙盐沉淀;形态观察可见矿化结节及其形成过程 结果 体外培养细胞显示成骨细胞的生物学特性.结论 这一体外培养的细胞模型的建立,为开展成骨细胞生物学的研究,探索骨质疏松的相关因子与骨质增生症的病因提供了基础.Objective Method for osteoblasts cultured in vitro was established and characterized. Methods Osetoblasts from new born rat cravarial bone were isolated by mechanical and enzymatic methods and, were cul-tured in DMEM medium with 10% fetal bovine serum. Results The osteoblasts showed alkaline phosphatase activ-ity and deposition of calcium salt with mineralized nodules. Conclusions The in vitro model of osteoblast can be served for the study of osteoblast biology, and probed for the related factors of osteoprosis and the genesis of osseous hyperplasia.

关 键 词：成骨细胞 结节 观察 体外培养细胞 骨质疏松 新生大鼠 骨质增生症 养成 生物学 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学] R681[医药卫生—基础医学]