APA微囊植入小鼠体内的生物相容性和急性毒性观察  

Observation about bio-campatibility and toxility of APA microcapsules transplantation into mouse

作　　者：李新建[1] 薛毅珑[1] 陆庆国[1] 李雁凌[1] 罗芸[1] 崔忻[1] 高宇红[1] 

机构地区：[1]解放军总医院老年医学研究所细胞生物室,北京100853

出　　处：《军医进修学院学报》2004年第5期378-379,共2页Academic Journal of Pla Postgraduate Medical School

基　　金：国家计委"九五"重点科技攻关基金资助项目 ( 95 12 0 5 0 )

摘　　要：目的 :了解植入型免疫隔离膜APA(海藻酸盐 多聚赖氨酸 海藻酸盐 )微囊的生物相容性和急性毒性。方法 :将不同剂量的APA微囊或APA微囊化牛肾上腺嗜铬细胞 (APA BCC)植入小鼠背部皮下或腹腔内 ,观察移植后不同时间微囊形态的变化 ,并观察移植后小鼠的日常活动、进食情况和体重 ,以断颈法将小鼠处死 ,取其心、肝、脾、肺、肾、小脑、小肠和肌肉等内脏器官。结果 :空微囊植入小鼠皮下或腹腔 4个月后大部分保持完好 ;APA BCCs植入小鼠背部皮下或腹腔 2个月后大部分微囊保持完好 ,但移植后 4个月部分微囊被包绕或破损 ;大剂量、超剂量植入APA微囊的情况下 ,小鼠的日常活动和进食情况均正常 ,14d时肉眼见小鼠腹腔内无明显异常 ,病理学检查主要器官未见异常改变。结论 :APA BCCs微囊材料对肌体无明显毒副作用 。Objective:To study the bio-compatible and toxic effects of the embeded immu-isolated membrane, the APA(alginate-polylysine-alginate) microcapsules. Methods:Transplanting different doses of APA microcapsules or APA microcapsulized bovine adrenal chromaffin cells(APA-BCC) into dorsal hypoderma or abdomen of the mouse, to observe changes of the microcapsule shape; to observe the normal activity, food taking and weight. Results:APA microcapsules mostly remained integrated 4 months after the transplantation; APA-BCC microcapsules mostly remained integrated after 2 months, but some were destroyed or enwraped after 4 months; The activity and food taking were normal after transplantation of high-dose or hyper-dose APA; There was no macroscopic abnormality in the abdomen and pathological changes in other important organs. Conclusion:APA microcapsule materials are remarkably harmless to bodies and of good bio-compatibility.

关 键 词：小鼠 植入 APA微囊 生物相容性 皮下 移植后 日常活动 

分 类 号：R392.12[医药卫生—免疫学] R730[医药卫生—基础医学]