高效液相色谱法测定人血浆中塞来昔布的浓度  

Determination of celecoxib in blood plasma by RP-HPLC with UV detection

作　　者：温预关[1] 蔡素华[1] 陈国中[1] 陈伟家[1] 

机构地区：[1]广州市脑科医院国家临床药理研究基地,广东广州510370

出　　处：《中国医院药学杂志》2004年第10期602-604,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立测定人血浆中塞来昔布浓度的高效液相色谱法。方法 :以美国迪马公司钻石C1 8反相柱 (2 5 0mm× 4 .6mm ,5 μm)为色谱柱 ,流动相为甲醇 水 (85∶1 5 ) ,流速为0 .8mL·min-1 ,检测波长 2 5 4nm ,以醋酸乙酯为提取剂。结果 :塞来昔布高、中、低 (80 0 .0 ,2 0 0 .0 ,2 5 .0 μg·L-1 ) 3种浓度的平均回收率分别为 1 0 0 .1 5 %,95 .31 %,92 .2 0 %,日内、日间差RSD均低于 6 %;分析方法的最低检测浓度为 1 0 .0 μg·L-1 ,线性范围为 2 5 .0～ 1 0 0 0 .0 μg·L-1 。结论 :该方法灵敏、准确、简单、快速 ,可用于临床血浓监测和药动学研究。OBJECTIVE To develop a HPLC method for quantitative determination of celecoxib in blood plasma. METHODS Blood plasma samples were extracted with hexane to remove lipid and interfering substances, then extracted with ethyl acetate. The residue was analyzed with a reverse phase HPLC system (C 18 column, 4.6 mm×250 mm, 5 μm; Mobile phase, MeOH-H 2O (85∶15); UV detection, 254 nm).RESULTS The average recoveries for celecoxib were 100.15%, 95.31% and 92.20%,respectively. The within-day and day-to-day relative standard deviations were less than 6%. The calibration curves had good linearity (r= 0.999 5) within a concentration range of 25.0- 1 000.0 μg·L -1. The limit of quantitation for celecoxib was 10.0 μg·L -1. CONCLUSION The method provides a sensitive, accurate, precise and reliable analytical procedure for clinical monitoring of celecoxib blood plasma and its phamacokinetic studies.

关 键 词：塞来昔布 人血浆 高效液相色谱法 药动学研究 临床 RSD 监测 公司 醋酸乙酯 美国 

分 类 号：R969.1[医药卫生—药理学] R927.2[医药卫生—药学]