甜瓜属种间杂交中3种不同倍性资源的微繁  

Micropropagation of Three Germplasms with Different Ploidies from Interspecific Hybridization in Cucumis

作　　者：罗向东[1] 陈劲枫[1] 钱春桃[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室园艺学院,南京210095

出　　处：《植物生理学通讯》2004年第5期549-552,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30170644);国家"863"计划项目(2002AA241251;2002AA207012;2004AA241120);教育部"跨世纪优秀人才培养计划";教育部科学技术研究重点项目(重点01097)。

摘　　要：以3种不同倍性的甜瓜属种间杂交资源(双二倍体Cucumis hytivus、异源三倍体黄瓜和种间杂种F1)无菌苗的顶芽或腋芽为试材,比较它们再生能力的差异,寻求各自增殖的最适培养基,从而建立其离体繁殖体系.结果表明:倍性越高,增殖能力越小,增殖速度也最小(双二倍体种转接的间隔时间比其它两种材料长1～2周).双二倍体、异源三倍体黄瓜和种间杂种F1的最大繁殖系数分别为4.6、7.1和8.4;相对应的培养基分别为MS+0.05 mg·L-1TDZ、MS+2.2 mg·L-1 6-BA和MS+0.5 mg·L-1 6-BA).3种材料的生根结果基本一致,在1/2MS+0.2 mg·L-1IBA上的生根率均能达84.5%,驯化成活率达90%以上.同一材料的田间性状表现整齐一致,没有发现变异.Shoot-tips of three germplasms with different ploidies from interspecific hybridization in Cucumis(amphidiploid, allotriploid and interspecific hybrid F1) were in vitro multiplied on Murashige-Skoog basal me-dium (MS) to compare their proliferate ability, and to investigate the effects of concentration of 6-benzyladenine(6-BA) and TDZ on their regeneration. The results indicated that the regeneration rates and speed decreased asthe ploidy level increased, e.g. it took 1~2 more week to subculture the amphidiploid than the others. Thehighest proliferation ratios obtained for the amphidiploid, allotriploid and the interspecific F1 hybrid were 4.6, 7.1and 8.4 respectively and the relevant media were MS+0.05 mgL-1 TDZ, MS+2.2 mgL-1 6-BA and MS+0.5mgL-1 6-BA, respectively. Over 84.5% shoots (0.5~1.0 cm) rooted on 1/2MS+0.2 mgL-1 IBA in 2 weeks forall germplasms, and more than 90% rooted shoots survived after acclimatization. There were not variationamong these acclimatized plantlets based on morphological observation and chromosome counts.

关 键 词：双二倍体 倍性 种间杂交 甜瓜 6-BA 种间杂种 黄瓜 属种 微繁 异源三倍体 

分 类 号：S652[农业科学—果树学]