植物生长调节剂对转基因鱼腥藻7120生长与外源基因表达的影响  

Effect of Plant Growth Substances on Growth and Exogenous Gene Expression of Recombinant Anabaena sp. PCC7120

作　　者：刘志伟[1] 张晨[1] 侯雨文[1] 郭勇[2] 

机构地区：[1]嘉应学院生物系,梅州514015 [2]华南理工大学食品与生物工程学院,广州510640

出　　处：《植物生理学通讯》2004年第5期567-569,共3页Plant Physiology Communications

基　　金：广东省自然科学基金项目(980541)。

摘　　要：适宜浓度的IBA、6-BA、赤霉酸和氯化胆碱均促进转基因鱼腥藻7120生长,提高其生物量,不影响外源基因表达,但从总体上讲高浓度植物生长调节剂则抑制藻细胞生长,降低外源基因表达水平。这些植物生长调节剂混合施用促进转基因鱼腥藻生长效果不如单一因子好,外源基因表达水平也略有下降。Appropriate concentration of indole-3-butyeic acid (IBA), 6-benzyladenine (6-BA), gibberelic acid(GA3) and choline chloride (CC) could promote cell growth of recombinant Anabaena sp. PCC7120, and hadno effect on exogenous gene expression. The high concentration of IBA, 6-BA, GA3 and CC would restrain cellgrowth and reduce exogenous gene expression. When IBA, 6-BA, GA3 and CC were added to medium together,the promotion to cell growth was weaker than the action of anyone plant growth substances, and exogenousgene expression level was reduced slightly.

关 键 词：植物生长调节剂 转基因鱼 生长效果 混合施用 6-BA 赤霉酸 氯化胆碱 外源基因表达 鱼腥藻 藻细胞 

分 类 号：S482.8[农业科学—农药学]