反相高效液相色谱法测定诺氟沙星锌干混悬剂的含量  

Determination of norfloxacin zinc for suspension by RP- HPLC

作　　者：王胜浩[1] 左军斌[1] 林芳[1] 

机构地区：[1]浙江省医学科学院药物研究所,浙江杭州310013

出　　处：《中国现代应用药学》2004年第5期387-388,共2页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省医药卫生优秀人才基金资助

摘　　要：目的　建立一种测定诺氟沙星锌 (NF Zn)干混悬剂含量的RP HPLC。方法　采用Lichrospher5 C18柱 (2 0 0mm× 4 .6mm) ,0 .0 2 5mol/L磷酸溶液 乙腈 (87∶13)为流动相 ,检测波长为 2 78nm ,流速为 0 .8mL/min。结果　该法能较好地测定NF Zn的含量。方法回收率为 (99.37± 0 .4 2 ) % ,最低检出限为 0 .2ng ,日间、日内RSD均小于 1.2 % ,在 11.0 1～ 137.6 0 μg/mL浓度范围内线性关系良好 ,线性方程为Y =- 1.6 816 5 +8.9315 4× 10 -6X ,r =0 .9998。结论　该法专属性好 ,简捷 ,快速准确 。OBJECTIVE To develop a RP-HPLC method for determination of norfloxacin zinc granule. METHOD A Lichrospher5-C_(18) column (200mm×4.6mm) , a mobile phase of phosphoric acid : acetonitrile (87∶13), and a UV detector of 278nm was used in the analysis, the flow rate was 0.8mL/min.RESULTS The linear range of the determination was 11.01～(137.6)μg/mL, the linear equation was Y=-1.68165+8.93154×10^(-6)X,the correlation coefficient was 0.9998,the recovery for this method was (99.37±0.42)% and the least detective limit was 0.2ng ,the intra-day and inter-day RSD were less than 1.2%.CONCLUSION The method established is specific,simple,rapid,accurate and suitable for the quality control of the norfloxacin zinc for suspension.

关 键 词：反相高效液相色谱法 诺氟沙星锌 干混悬剂 含量测定 

分 类 号：R917.01[医药卫生—药物分析学] R978.27[医药卫生—药学]